Abstract |
Although superoxide dismutase (SOD) has been reported to promote functional recovery in ischemic spinal cord injury, it presents many difficulties in practical use primarily due to its short half-life in vivo and low tissue affinity. In this study, we investigated the effects of a new type of SOD, a lecithinized superoxide dismutase (PC-SOD), on motor disturbances, spinal cord edema, levels of myeloperoxidase (MPO), and spinal cord blood flow (SCBF) after spinal cord injury (SCI) in rats. PC-SOD is reported to show a delayed plasma disappearance in vivo in rats and has a higher affinity for vascular endothelium cells, neutrophils, and other cells than unmodified SOD. PC-SOD (4000 units/kg), unmodified SOD (4000 units/kg), or vehicle was injected intravenously 30 min after SCI. Four hours after SCI, SOD activities in spinal cord tissue and plasma were significantly higher in the PC-SOD group than in the unmodified SOD group. In the PC-SOD-treated rats, motor function was significantly better than in the other 2 groups of rats. PC-SOD significantly suppressed MPO activity, an indicator of neutrophils infiltration, in the spinal cord, at 4, 8, and 24 h after SCI, and spinal cord edema at 24 h after SCI. Moreover, the decrease of SCBF after SCI was less marked in the PC-SOD group. The present results suggest that lecithinization can improve the drug delivery of SOD to the spinal cord and PC-SOD may be an alternative pharmacological treatment for SCI.
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Authors | K Nakauchi, T Ikata, S Katoh, Y Hamada, K Tsuchiya, K Fukuzawa |
Journal | Journal of neurotrauma
(J Neurotrauma)
Vol. 13
Issue 10
Pg. 573-82
(Oct 1996)
ISSN: 0897-7151 [Print] United States |
PMID | 8915909
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Phosphatidylcholines
- Peroxidase
- Superoxide Dismutase
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Topics |
- Animals
- Edema
(drug therapy, physiopathology)
- Male
- Movement
(physiology)
- Peroxidase
(antagonists & inhibitors, metabolism)
- Phosphatidylcholines
- Rats
- Rats, Wistar
- Regional Blood Flow
(drug effects, physiology)
- Spinal Cord
(blood supply, enzymology)
- Spinal Cord Injuries
(drug therapy, enzymology, physiopathology)
- Superoxide Dismutase
(administration & dosage, metabolism, therapeutic use)
- Time Factors
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