HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Production of T-helper cell subsets and cytokines by lymphocytes from patients with chronic mucocutaneous candidiasis.

Abstract
Chronic mucocutaneous candidiasis (CMC) is a heterogeneous group of disorders characterized by recurrent and persistent superficial candidal infections. Cytokine-induced dysregulation of T-helper cell function has been described in other immune-deficient states but has not been studied in CMC patients. We studied T-helper cell subsets by flow cytometry and cytokine production by stimulated lymphocytes in six CMC patients, two healthy pediatric controls, and five healthy adult controls. Peripheral blood lymphocytes were stimulated in vitro with phytohemagglutinin or Candida albicans extract, and the production of interleukin-2R (IL-2R), IL-4, IL-10, and gamma interferon in the supernatants was measured by enzyme-linked immunosorbent assay. CMC patients had a decrease in the CD29+/CD29+ cell population compared with the numbers in controls (P < 0.02). The percentage of CD4+/CD45RA+ cells was greater in patients than in controls, but the difference was not significant. There was no difference in the production of IL-10 or gamma interferon by the patient lymphocytes. CMC patients produced more IL-4 than the controls (P < 0.001), whereas the controls tended to produce more IL-2R than the patients (P = 0.19). These findings support the concept that a decrease in CD4+/CD29+ T-helper inducer cells along with T-helper cell dysregulation may lead to defective memory responses to antigens in CMC patients and a decrease in cell-mediated immunity due to inhibition of TH1 cells by increased levels of IL-4.
AuthorsL J Kobrynski, L Tanimune, L Kilpatrick, D E Campbell, S D Douglas
JournalClinical and diagnostic laboratory immunology (Clin Diagn Lab Immunol) Vol. 3 Issue 6 Pg. 740-5 (Nov 1996) ISSN: 1071-412X [Print] United States
PMID8914768 (Publication Type: Journal Article)
Chemical References
  • Cytokines
Topics
  • Adult
  • Candidiasis, Chronic Mucocutaneous (immunology)
  • Child
  • Child, Preschool
  • Cytokines (biosynthesis)
  • Female
  • Humans
  • Lymphocyte Activation (immunology)
  • Male
  • T-Lymphocyte Subsets (immunology, metabolism)
  • T-Lymphocytes, Helper-Inducer (immunology, metabolism)
  • Th1 Cells (immunology, metabolism)
  • Th2 Cells (immunology, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: