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Fucosyltransferase III and sialyl-Le(x) expression correlate in cultured colon carcinoma cells but not in colon carcinoma tissue.

Abstract
The potential contribution of fucosyltransferases to the overexpression of sialyl-Le(x) antigen was investigated in the colon carcinoma cell line HT-29 and in human colon carcinoma tissue. In HT-29 cells as well as in normal or malignant colonic tissues Fuc-TIII, Fuc-TIV, Fuc-TVI but not Fuc-TV nor Fuc-TVII were detectable after RT-PCR. Sodium butyrate treatment of HT-29 cells increased (to about 200%) and DMSO treatment decreased (to about 20%) the expression of sialyl-Le(x). This modulation of sialyl-Le(x) was concomitant with the analogous increase/decrease of mRNA of Fuc-TIII but not Fuc-TIV. Fuc-TVI was not detectable by Northern blotting in HT-29 cells. In six human colon carcinomas which exhibited strong overexpression of sialyl-Le(x), the expression of Fuc-TIII-mRNA was the same or lower than in the corresponding normal colonic tissue. Thus Fuc-TIII expression may be affecting the expression of the sialyl-Le(x) moiety in HT-29 cells but not in human colon carcinoma tissue.
AuthorsC Hanski, E Klussmann, J Wang, C Böhm, D Ogorek, M L Hanski, S Krüger-Krasagakes, J Eberle, A Schmitt-Gräff, E O Riecken
JournalGlycoconjugate journal (Glycoconj J) Vol. 13 Issue 5 Pg. 727-33 (Oct 1996) ISSN: 0282-0080 [Print] United States
PMID8909999 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Monoclonal
  • Butyrates
  • Lewis X Antigen
  • RNA, Messenger
  • Butyric Acid
  • Fucosyltransferases
  • Dimethyl Sulfoxide
Topics
  • Antibodies, Monoclonal (immunology, metabolism)
  • Blotting, Northern
  • Butyrates (pharmacology)
  • Butyric Acid
  • Colonic Neoplasms (enzymology, metabolism)
  • Dimethyl Sulfoxide (pharmacology)
  • Fucosyltransferases (metabolism)
  • Gene Expression Regulation, Neoplastic (genetics)
  • Humans
  • Lewis X Antigen (biosynthesis, metabolism)
  • Polymerase Chain Reaction
  • RNA, Messenger (metabolism)
  • Tumor Cells, Cultured

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