Abstract |
The potential contribution of fucosyltransferases to the overexpression of sialyl-Le(x) antigen was investigated in the colon carcinoma cell line HT-29 and in human colon carcinoma tissue. In HT-29 cells as well as in normal or malignant colonic tissues Fuc-TIII, Fuc-TIV, Fuc-TVI but not Fuc-TV nor Fuc-TVII were detectable after RT-PCR. Sodium butyrate treatment of HT-29 cells increased (to about 200%) and DMSO treatment decreased (to about 20%) the expression of sialyl-Le(x). This modulation of sialyl-Le(x) was concomitant with the analogous increase/decrease of mRNA of Fuc-TIII but not Fuc-TIV. Fuc-TVI was not detectable by Northern blotting in HT-29 cells. In six human colon carcinomas which exhibited strong overexpression of sialyl-Le(x), the expression of Fuc-TIII- mRNA was the same or lower than in the corresponding normal colonic tissue. Thus Fuc-TIII expression may be affecting the expression of the sialyl-Le(x) moiety in HT-29 cells but not in human colon carcinoma tissue.
|
Authors | C Hanski, E Klussmann, J Wang, C Böhm, D Ogorek, M L Hanski, S Krüger-Krasagakes, J Eberle, A Schmitt-Gräff, E O Riecken |
Journal | Glycoconjugate journal
(Glycoconj J)
Vol. 13
Issue 5
Pg. 727-33
(Oct 1996)
ISSN: 0282-0080 [Print] United States |
PMID | 8909999
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antibodies, Monoclonal
- Butyrates
- Lewis X Antigen
- RNA, Messenger
- Butyric Acid
- Fucosyltransferases
- Dimethyl Sulfoxide
|
Topics |
- Antibodies, Monoclonal
(immunology, metabolism)
- Blotting, Northern
- Butyrates
(pharmacology)
- Butyric Acid
- Colonic Neoplasms
(enzymology, metabolism)
- Dimethyl Sulfoxide
(pharmacology)
- Fucosyltransferases
(metabolism)
- Gene Expression Regulation, Neoplastic
(genetics)
- Humans
- Lewis X Antigen
(biosynthesis, metabolism)
- Polymerase Chain Reaction
- RNA, Messenger
(metabolism)
- Tumor Cells, Cultured
|