Abstract |
Liver sections from aging ad libitum-fed and diet-restricted B6C3F1 male mice were evaluated immunohistochemically for pi-class glutathione S-transferase ( GST-II). GST-II immunostaining of hepatocytes was diffuse and occurred in periportal regions of hepatic acinus, whereas perivenous areas were weakly stained or were stain-free. Expression of GST-II was significantly diminished in diet-restricted mice in all age groups and was associated with a marked decrease in liver tumor development. As most spontaneous liver tumors were GST-II positive, it can be speculated that they developed from GST-II positive initiated hepatocytes. To determine whether dietary restriction induces apoptosis in GST-II-positive hepatocytes, 24-month-old ad libitum-fed mice were introduced to 40% diet restriction. After 1 week of diet restriction, a decrease in GST-II expression was associated with a threefold increase in the frequency of apoptotic bodies as detected by terminal deoxynucleotidyl transferase-mediated d- UTP nick end labeling of DNA fragments. A two-step immunohistochemical procedure revealed that approximately 70% of apoptotic bodies were GST-II positive. These results suggest that spontaneous, potentially preneoplastic hepatocytes in tumor-prone B6C3F1 mice are eliminated by apoptosis with dietary restriction.
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Authors | L Muskhelishvili, A Turturro, R W Hart, S J James |
Journal | The American journal of pathology
(Am J Pathol)
Vol. 149
Issue 5
Pg. 1585-91
(Nov 1996)
ISSN: 0002-9440 [Print] United States |
PMID | 8909248
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
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Topics |
- Aging
(pathology)
- Animals
- Apoptosis
- Cells, Cultured
- Female
- Food Deprivation
- Glutathione Transferase
(analysis, classification)
- Immunohistochemistry
- Liver
(enzymology, pathology)
- Male
- Mice
- Mice, Inbred C3H
- Mice, Inbred C57BL
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