Sialyl Lewis X analog inhibits eosinophil accumulation and late asthmatic response in a guinea-pig model of asthma.

Abstract

Preferential eosinophil accumulation is characteristic of airway inflammation in asthma. Although little is known about its mechanism, the effect of a sialyl Lewis X analog on airway eosinophilia was examined in a guinea-pig model of asthma. Guinea-pigs were sensitized by repeated inhalation of ovalbumin. After a single inhalation challenge, the animals showed striking airway eosinophilia and a late asthmatic response. In contrast, when guinea-pigs were pretreated intravenously with sialyl Lewis X analog (LX 0104) 1 h before antigen challenge, both eosinophil infiltration in the tracheal wall and the late asthmatic response were significantly inhibited in a dose-dependent manner. In a further in vitro study, LX 0104 significantly suppressed the adhesion of human and guinea-pig eosinophils to human umbilical vein endothelial cells activated with interleukin-1 beta. These results suggest that LX 0104 plays a critical role in antigen-induced airway eosinophilia and the late asthmatic response.

• Authors: H Sagara, C Ra, T Okada, S Shinohara, T Fukuda, K Okumura, S Makino
• Journal: International archives of allergy and immunology (Int Arch Allergy Immunol) Vol. 111 Suppl 1 Pg. 32-6 ( 1996) ISSN: 1018-2438 [Print] Switzerland
• PMID: 8906110 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• LX 0104
• Oligosaccharides
• Sialyl Lewis X Antigen

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Asthma (drug therapy)
• Cell Adhesion (drug effects)
• Cells, Cultured
• Chemotaxis, Leukocyte (drug effects)
• Cross Reactions
• Endothelium, Vascular (cytology)
• Eosinophils (immunology)
• Guinea Pigs
• Humans
• Male
• Oligosaccharides (pharmacology)
• Sialyl Lewis X Antigen
• Time Factors