Abstract |
A 2.0-kbp Pst I DNA fragment of the unicellular cyanobacterium Synechococcus sp. strain PCC 6301 genome contains two open reading frames (ORFs). The first ORF of 100 codons potentially encodes a polypeptide having 47% amino acid identity to Escherichia coli ribosomal protein S14, suggesting it as a ribosomal protein S14 gene (rps14). The second ORF of 351 codons is located 81 bp downstream of rps14 and its deduced amino acid sequence is in part similar to that of the Salmonella typhimurium oligopeptide permease membrane protein OppC. Northern blot analysis showed that rps14 is expressed as a 0.48-kb transcript whereas no transcript was detected from ORF351. Pulsed-field electrophoresis and blot hybridization analysis revealed that rps14 is a single-copy gene and is found within a 165-kbp region located upstream of rrnA on the circular genome.
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Authors | T Fujishiro, T Kaneko, M Sugiura, M Sugita |
Journal | DNA research : an international journal for rapid publication of reports on genes and genomes
(DNA Res)
Vol. 3
Issue 3
Pg. 165-9
(Jun 30 1996)
ISSN: 1340-2838 [Print] England |
PMID | 8905234
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Bacterial Proteins
- Carrier Proteins
- DNA, Bacterial
- Membrane Proteins
- Membrane Transport Proteins
- OppC-like protein, Synechococcus sp.
- Ribosomal Proteins
- ribosomal protein S14
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Topics |
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Carrier Proteins
- Chromosome Mapping
- Chromosomes, Bacterial
- Cyanobacteria
(genetics, metabolism)
- DNA, Bacterial
- Membrane Proteins
(genetics)
- Membrane Transport Proteins
- Molecular Sequence Data
- Multigene Family
- Ribosomal Proteins
(genetics)
- Sequence Homology, Amino Acid
- Transcription, Genetic
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