Characterization of manganese peroxidases from the hyperlignolytic fungus IZU-154.

Abstract

Four isozymes of manganese peroxidase (MnP) were identified in the culture fluid of the hyperlignolytic fungus IZU-154 under nitrogen starvation conditions. One of them was purified and characterized kinetically. The specific activity and Kcat/K(m) value of the MnP from IZU-154 were 1.6 times higher than those of the MnP from a typical lignin-degrading fungus, Phanerochaete chrysosporium. Two cDNAs encoding MnP isozymes from IZU-154 were isolated. The coding sequence of the two cDNAs, IZ-MnP1 cDNA and IZ-MnP2 cDNA, were 1,152 (384 amino acids) and 1,155 (385 amino acids) bp in length, respectively. They exhibit 96.2% identity at the nucleotide level and 95.1% identity at the amino acid level. Southern blot analysis indicated that two MnP isozyme genes exist in IZU-154 genomic DNA. The primary structures of two MnPs from IZU-154 were similar to those of MnPs from P. chrysosporium. The amino acid sequences including the important residues identified in MnPs from P. chrysosporium, such as the manganese-binding residues, the calcium-binding residues, the disulfide bonds, and the N-glycosylation site, were conserved in the two deduced IZ-MnPs. However, several discrepancies were found in the context around the distal histidine residue between MnP from IZU-154 and MnP from P. chrysosporium, which likely led to the difference in the kinetic parameters for MnP function.

• Authors: M Matsubara, J Suzuki, T Deguchi, M Miura, Y Kitaoka
• Journal: Applied and environmental microbiology (Appl Environ Microbiol) Vol. 62 Issue 11 Pg. 4066-72 (Nov 1996) ISSN: 0099-2240 [Print] United States
• PMID: 8899997 (Publication Type: Comparative Study, Journal Article)

Chemical References

• DNA Primers
• DNA, Complementary
• DNA, Fungal
• Isoenzymes
• Lignin
• Peroxidases
• manganese peroxidase

Topics

• Amino Acid Sequence
• Base Sequence
• Basidiomycota (enzymology, genetics, metabolism)
• Binding Sites (genetics)
• Conserved Sequence
• DNA Primers (genetics)
• DNA, Complementary (genetics)
• DNA, Fungal (genetics)
• Isoenzymes (genetics, isolation & purification, metabolism)
• Kinetics
• Lignin (metabolism)
• Molecular Sequence Data
• Peroxidases (genetics, isolation & purification, metabolism)
• Restriction Mapping
• Sequence Homology, Amino Acid
• Species Specificity