The activity of the gluconate-H+ symporter of Schizosaccharomyces pombe cells is down-regulated by D-glucose and exogenous cAMP.

Abstract

Schizosaccharomyces pombe cells take up D-gluconate, as an alternative carbon source for growth, during glucose starvation or when cultured on glycerol-containing medium. Gluconate uptake is not detectable while cells are growing logarithmically on glucose. The addition of D-glucose as well as its non-metabolizable analogues to glycerol-grown cells causes an immediate loss of gluconate transport within 1 min. The reversible down-regulation of the gluconate carrier occurs after glucose has been internalized. This regulation is triggered not only by D-glucose but also by extracellular cAMP even in the absence of the cAMP-dependent protein kinase (PKA1).

• Authors: T Caspari, S Urlinger
• Journal: FEBS letters (FEBS Lett) Vol. 395 Issue 2-3 Pg. 272-6 (Oct 21 1996) ISSN: 0014-5793 [Print] England
• PMID: 8898110 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Carrier Proteins
• Gluconates
• Cyclic AMP
• Cyclic AMP-Dependent Protein Kinases
• Glucose
• gluconic acid

Topics

• Biological Transport
• Carrier Proteins (metabolism)
• Cyclic AMP (pharmacology)
• Cyclic AMP-Dependent Protein Kinases (metabolism)
• Gluconates (metabolism)
• Glucose (analogs & derivatives, metabolism, pharmacology)
• Kinetics
• Schizosaccharomyces (drug effects, metabolism)