Involvement of hydrogen peroxide production in erbstatin-induced apoptosis in human small cell lung carcinoma cells.

Abstract

Tyrosine kinase inhibitor, erbstatin, induced morphological apoptosis and DNA fragmentation in human small cell lung carcinoma (SCLC) cells. Erbstatin-induced apoptosis was inhibited by antioxidants, whereas erbstatin-inhibited tyrosine phosphorylation was not affected by them. Erbstatin was shown by means of flow cytometry to induce hydrogen peroxide generation. Furthermore, hydrogen peroxide induced morphological apoptosis and DNA fragmentation in the SCLC cells. We also demonstrated that erbstatin-induced hydrogen peroxide production and DNA fragmentation were partially suppressed by inhibition of protein synthesis. Thus, erbstatin-induced apoptosis would be due to hydrogen peroxide generation via newly synthesized protein.

• Authors: S Simizu, M Imoto, N Masuda, M Takada, K Umezawa
• Journal: Cancer research (Cancer Res) Vol. 56 Issue 21 Pg. 4978-82 (Nov 01 1996) ISSN: 0008-5472 [Print] United States
• PMID: 8895753 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Enzyme Inhibitors
• Hydroquinones
• Cycloheximide
• Hydrogen Peroxide
• Protein-Tyrosine Kinases
• erbstatin

Topics

• Antioxidants (pharmacology)
• Apoptosis (drug effects)
• Carcinoma, Small Cell (metabolism, pathology)
• Cycloheximide (pharmacology)
• Enzyme Inhibitors (pharmacology)
• Humans
• Hydrogen Peroxide (metabolism, toxicity)
• Hydroquinones (pharmacology)
• Lung Neoplasms (metabolism, pathology)
• Protein-Tyrosine Kinases (antagonists & inhibitors)
• Tumor Cells, Cultured