Abstract | BACKGROUND: METHODS: We collected thyroid cancer tissues from 93 patients with thyroid cancer for developing the primary culture. Thyroid cancer tissues were verified by frozen sections during the operations, then subjected to primary cell culture. During the first several passages, fibroblasts were removed by selective attachment. RESULTS: From the 93 cancer tissues used, two follicular cancer tissues from the metastatic area ( CGTH W-1, CGTH W-2) and one papillary thyroid cancer tissue (CGTH W-3) could be passed over 50 times. Cellular transformation with loss of contact inhibition occurred during passages 5-8. Electron microscopic studies of the CGTH W-1 cell line showed the presence of an abundance of mitochondria and Golgi complex. Presence of microvilli with interdigitations between neighboring cells were found in CGTH W-2 and CGTH W-3 cell lines. Severe combined immunodeficient (SCID) mice were used to determine whether these cells were tumorigenic. Two months after transplantation of 1 x 10(7) CGTH W-1, 2, and 3 cells into SCID mice, subcutaneous tumors approximately 2-2.5 cm in size were clearly visible. After affinity cross-linking an IGF-I with insulin-like growth factor binding proteins complex corresponding to a molecular size of 41 kDa was observed in culture media collected from CGTH W-1, SW 579, and RO82 W-1 cells. CONCLUSIONS:
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Authors | J D Lin, T C Chao, H F Weng, H S Huang, Y S Ho |
Journal | Journal of surgical oncology
(J Surg Oncol)
Vol. 63
Issue 2
Pg. 112-8
(Oct 1996)
ISSN: 0022-4790 [Print] United States |
PMID | 8888804
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Culture Media
- Thyroglobulin
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Topics |
- Animals
- Carcinoma
- China
- Culture Media
(analysis)
- Humans
- Mice
- Mice, SCID
- Thyroglobulin
(analysis)
- Thyroid Neoplasms
- Transplantation, Heterologous
- Tumor Cells, Cultured
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