Cytotoxicity of two new ribosome-inactivating proteins, cinnamomin and camphorin, to carcinoma cells.

Abstract

Cinnamomin (two-chain) and camphorin (single-chain), two novel ribosome-inactivating proteins (RIPs) purified from the seeds of Cinnamomum camphora, produced inhibitory effects in cultured carcinoma cells. The IC50 of cinnamomin to the human hepatocarcinoma cell-line 7721 and the melanoma cell-line M21 were 18.8 nmol and 11.7 nmol respectively. The IC50 of camphorin to the human hepatocarcinoma cell-line 7721 was 59 nmol, whereas the melanoma cell-line M21 was not susceptible to camphorin. Furthermore, cinnamomin exhibited a remarkable inhibitory effect on the growth of solid melanoma in the skin of the nude mouse. An R-fragment could be isolated from ribosomes of cinnamomin- or camphorin-treated carcinoma cells after incubation with acidic aniline, indicating that the cytotoxicity of these two new RIPs to carcinoma cells might result from modification to the ribosomes.

• Authors: J Ling, W Y Liu
• Journal: Cell biochemistry and function (Cell Biochem Funct) Vol. 14 Issue 3 Pg. 157-61 (Sep 1996) ISSN: 0263-6484 [Print] England
• PMID: 8888568 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Algal Proteins
• Antineoplastic Agents
• DNA, Superhelical
• Fungal Proteins
• Plant Proteins
• Proteins
• Ribosome Inactivating Proteins, Type 2
• camphorin protein, Cinnamomum camphora
• cinnamomin, Phytophthora cinnamomi
• N-Glycosyl Hydrolases
• Ribosome Inactivating Proteins

Topics

• Algal Proteins
• Animals
• Antineoplastic Agents (pharmacology)
• Carcinoma, Hepatocellular (drug therapy)
• DNA, Superhelical (metabolism)
• Disease Susceptibility
• Fungal Proteins (pharmacology)
• Humans
• Melanoma (drug therapy)
• Mice
• Mice, Nude
• N-Glycosyl Hydrolases (metabolism)
• Plant Proteins (pharmacology)
• Proteins
• Ribosome Inactivating Proteins
• Ribosome Inactivating Proteins, Type 2
• Ribosomes
• Tumor Cells, Cultured