Abstract |
Expression of seven serotonin or 5-hydroxytryptamine (5-HT) receptors (5-HT1D alpha, 5-HT1E, 5-HT2, 5-HT1A, 5-HT1C, 5-HT1D beta, and 5-HT6) was investigated in human normal fetal astrocytes and eight glioma cell lines by reverse transcription and polymerase chain reaction (RT-PCR). No expression of 5-HT1D beta and 5-HT6 was observed in any of the cell lines studied. The 5-HT1D alpha receptor was found to be expressed in two human glioma cell lines but not in normal astrocytes. In addition, only three glioma cell lines expressed the 5-HT1E receptor. The 5-HT1C receptor was expressed in six glioma cell lines but not in normal astrocytes while the 5-HT1A was found to be expressed in normal astrocytes from the left hemisphere and in six glioma cell lines but not in normal astrocytes from the cerebellum. Interestingly, the 5-HT2 receptor was expressed in all cells studied but very weakly in normal astrocytes. The effect of 5-HT on glioma cell proliferation, migration, and invasion was also investigated. Serotonin was found to positively modulate these three processes in vitro. These results suggest that 5-HT may play an important role in the control of the biological properties of human glioma cells.
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Authors | A Merzak, S Koochekpour, M P Fillion, G Fillion, G J Pilkington |
Journal | Brain research. Molecular brain research
(Brain Res Mol Brain Res)
Vol. 41
Issue 1-2
Pg. 1-7
(Sep 05 1996)
ISSN: 0169-328X [Print] Netherlands |
PMID | 8883928
(Publication Type: Journal Article)
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Chemical References |
- Fetal Proteins
- Neoplasm Proteins
- Nerve Tissue Proteins
- RNA, Messenger
- RNA, Neoplasm
- Receptors, Serotonin
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Topics |
- Astrocytes
(drug effects, metabolism)
- Brain Neoplasms
(metabolism)
- Cell Division
(drug effects)
- Cell Movement
(drug effects)
- Fetal Proteins
(biosynthesis, genetics)
- Gene Expression Regulation, Neoplastic
- Glioma
(classification, metabolism, pathology)
- Humans
- Neoplasm Invasiveness
(physiopathology)
- Neoplasm Proteins
(biosynthesis, genetics)
- Nerve Tissue Proteins
(biosynthesis, genetics)
- Polymerase Chain Reaction
- RNA, Messenger
(analysis)
- RNA, Neoplasm
(analysis)
- Receptors, Serotonin
(biosynthesis, classification, drug effects, genetics)
- Tumor Cells, Cultured
(drug effects)
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