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Expression of serotonin receptors in human fetal astrocytes and glioma cell lines: a possible role in glioma cell proliferation and migration.

Abstract
Expression of seven serotonin or 5-hydroxytryptamine (5-HT) receptors (5-HT1D alpha, 5-HT1E, 5-HT2, 5-HT1A, 5-HT1C, 5-HT1D beta, and 5-HT6) was investigated in human normal fetal astrocytes and eight glioma cell lines by reverse transcription and polymerase chain reaction (RT-PCR). No expression of 5-HT1D beta and 5-HT6 was observed in any of the cell lines studied. The 5-HT1D alpha receptor was found to be expressed in two human glioma cell lines but not in normal astrocytes. In addition, only three glioma cell lines expressed the 5-HT1E receptor. The 5-HT1C receptor was expressed in six glioma cell lines but not in normal astrocytes while the 5-HT1A was found to be expressed in normal astrocytes from the left hemisphere and in six glioma cell lines but not in normal astrocytes from the cerebellum. Interestingly, the 5-HT2 receptor was expressed in all cells studied but very weakly in normal astrocytes. The effect of 5-HT on glioma cell proliferation, migration, and invasion was also investigated. Serotonin was found to positively modulate these three processes in vitro. These results suggest that 5-HT may play an important role in the control of the biological properties of human glioma cells.
AuthorsA Merzak, S Koochekpour, M P Fillion, G Fillion, G J Pilkington
JournalBrain research. Molecular brain research (Brain Res Mol Brain Res) Vol. 41 Issue 1-2 Pg. 1-7 (Sep 05 1996) ISSN: 0169-328X [Print] Netherlands
PMID8883928 (Publication Type: Journal Article)
Chemical References
  • Fetal Proteins
  • Neoplasm Proteins
  • Nerve Tissue Proteins
  • RNA, Messenger
  • RNA, Neoplasm
  • Receptors, Serotonin
Topics
  • Astrocytes (drug effects, metabolism)
  • Brain Neoplasms (metabolism)
  • Cell Division (drug effects)
  • Cell Movement (drug effects)
  • Fetal Proteins (biosynthesis, genetics)
  • Gene Expression Regulation, Neoplastic
  • Glioma (classification, metabolism, pathology)
  • Humans
  • Neoplasm Invasiveness (physiopathology)
  • Neoplasm Proteins (biosynthesis, genetics)
  • Nerve Tissue Proteins (biosynthesis, genetics)
  • Polymerase Chain Reaction
  • RNA, Messenger (analysis)
  • RNA, Neoplasm (analysis)
  • Receptors, Serotonin (biosynthesis, classification, drug effects, genetics)
  • Tumor Cells, Cultured (drug effects)

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