Novel mitochondrial proteins and decreased intrinsic activity of cytochrome-c oxidase. Characteristics of swayback disease in sheep.

Abstract

Swayback disease (SD), a fatal neurological disorder affecting lambs and kids, is characterized by abnormal mitochondria and low cytochrome-c oxidase activity. The cytochrome-c oxidase content and activity and the protein composition of mitochondria isolated from the brains of SD lambs were investigated. Difference spectra analysis indicated that the cytochrome-c oxidase content of mitochondria from SD animals was lower than normal, and electrophoresis showed that when compared to mitochondria from normal animals, lipid-depleted mitochondria from SD lambs had a different protein composition, particularly, in the 40-55 kDa region. Polarographic studies, using cytochrome-c as substrate, confirmed low intrinsic activity of cytochrome-c oxidase within the mitochondria of SD lambs. These studies also showed that at low ionic strength, such mitochondria did not yield the expected characteristic biphasic Eadie-Hofstee plots.

• Authors: T Alleyne, A Adogwa, A Lalla, J Joseph, R John
• Journal: Molecular and chemical neuropathology (Mol Chem Neuropathol) 1996 May-Aug Vol. 28 Issue 1-3 Pg. 285-93 ISSN: 1044-7393 [Print] United States
• PMID: 8871971 (Publication Type: Journal Article)

Chemical References

• Proteins
• Electron Transport Complex IV

Topics

• Animals
• Brain (metabolism)
• Electron Transport Complex IV (metabolism)
• Mitochondria (metabolism)
• Oxidation-Reduction
• Polarography
• Proteins (isolation & purification, metabolism)
• Reference Values
• Sheep
• Swayback (metabolism)