Abstract |
The DNA-breaking activity and cytotoxicity of docosahexaenoic acid ethyl ester ( DHAE) and its degradation products (dDHAE) induced by ultraviolet (UV) irradiation, were demonstrated in this study. The major component of dDHAE was identified as 4-hydroxyvaleric acid ethyl ester. DHAE and dHAE solutions at a concentration of 100 mg/ml induced single-strand breaks of plasmid DNA after an incubation at 37 degrees C and pH 7.6 for 15 hours. DHAE transformed a plasmid supercoiled DNA (Form I) into an open circular relaxed form (Form II), and dDHAE completely destroyed DNA molecules. HSC-4 cells, which were derived from human prickle cell carcinoma, were cultured with various concentrations (10, 30 and 50 micrograms/ml) of DHAE and dDHAE. Both inhibited the proliferation of HSC-4 cells. The potency of their cytotoxicity was dependent on their concentration. It is noteworthy that the lethal effects of DHAE and dDHAE on HSC-4 cells were quite similar, although the DNA-breaking activity of dDHAE was much greater than that of DHAE. These results suggested that DHAE becomes more cytotoxic after undergoing oxidization and metabolism in the cell.
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Authors | W Liu, H Nishio, T Mio, K Sumino |
Journal | The Kobe journal of medical sciences
(Kobe J Med Sci)
Vol. 41
Issue 6
Pg. 235-45
(Dec 1995)
ISSN: 0023-2513 [Print] Japan |
PMID | 8869009
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- DNA, Neoplasm
- Docosahexaenoic Acids
- 4,7,10,13,16,19-docosahexaenoic acid ethyl ester
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Topics |
- Antineoplastic Agents
(pharmacology)
- Cell Death
- DNA Damage
- DNA, Neoplasm
(drug effects)
- Docosahexaenoic Acids
(metabolism, pharmacology)
- Humans
- Tumor Cells, Cultured
(drug effects)
- Ultraviolet Rays
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