Protection against reperfusion-induced arrhythmias by human thioredoxin.

Abstract

Adult T-cell leukemia-derived factor (ADF), identified in the supernatant of adult T-cell leukemia (ATL) cell culture, is a human homologue of thioredoxin and consists of 104 amino acids; it has two redox-active half-cysteine residues in an exposed active center. Human thioredoxin has many biological activities, including growth promotion, cell activation, and a catalase-like radical scavenging activity. We examined the protective effect of human thioredoxin (h-thioredoxin) against reperfusion-induced arrhythmias in an isolated rat heart model with 10-min regional ischemia followed by 30-min reperfusion. Male Wistar rats were assigned to six groups: a control, a superoxide dismutase (SOD 8 x 10(4) IU/L), and a catalase group (1 x 10(6) IU/L), and three groups treated with h-thioredoxin [approximately .01 microM (TRX-I group), approximately 0.1 microM (TRX-II group), and approximately 1 microM (TRX-III group)]. In the early reperfusion period, h-thioredoxin reduced the incidence of ventricular fibrillation (VF) to 8% in the TRX-II group (p < 0.01) from the control value of 75%. SOD and catalase reduced the incidence of VF to 43 and 33%, respectively (NS). During the entire reperfusion period, the incidence of VF in the SOD group was 79%, as compared to 83% in the control group. In the catalase and TRX-II groups, the incidence of VF was significantly reduced to 42 and 25%, respectively. These findings indicate that SOD failed to protect against the reperfusion-induced arrhythmias. h-Thioredoxin exerted a protective effect against these arrhythmias; a concentration of approximately 0.1 micro was the most effective.

• Authors: M Aota, K Matsuda, N Isowa, H Wada, J Yodoi, T Ban
• Journal: Journal of cardiovascular pharmacology (J Cardiovasc Pharmacol) Vol. 27 Issue 5 Pg. 727-32 (May 1996) ISSN: 0160-2446 [Print] United States
• PMID: 8859944 (Publication Type: Journal Article)

Chemical References

• Recombinant Proteins
• Thioredoxins
• Catalase
• Superoxide Dismutase

Topics

• Animals
• Arrhythmias, Cardiac (prevention & control)
• Catalase (pharmacology)
• Humans
• Male
• Myocardial Reperfusion Injury (prevention & control)
• Rats
• Rats, Wistar
• Recombinant Proteins (pharmacology)
• Superoxide Dismutase (pharmacology)
• Thioredoxins (pharmacology)