HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

An immunohistochemical and immunoelectron microscopic study of adhesion molecules in synovial pannus formation in rheumatoid arthritis.

Abstract
To investigate the mechanism of synovial pannus formation in rheumatoid arthritis, immunohistochemical and immunoelectron microscopic studies with monoclonal antibodies against the adhesion molecules, CD54 (ICAM-1), CD11a (LFA-1), CDw49a (VLA-1), CDw49b (VLA-2), CDw49c (VLA-3), Cdw49d (VLA-4) and CDw49e (VLA-5), were carried out to determine the pattern of distribution of these molecules at the rheumatoid synovial cartilage junction. Treatment with anti-ICAM-1 resulted in membrane staining of most of the macrophages and fibroblasts infiltrating the synovial tissue and bordering and pannus-cartilage junction, suggesting the possibility that ICAM-1 may function to facilitate the adhesion of synovial type A cells bearing ICAM-1 to type B cells of the pannus. ICAM-1 positive macrophages and fibroblasts were often found to be in contact with lymphoid cells, suggesting also that a cellular immune reaction occurs in the formation of the pannus. In addition, VLA-3, VLA-4 and, particularly, VLA-5 were the predominant beta 1 integrins expressed by rheumatoid synovial pannus. Sine these three integrins all function as fibronectin receptors, it is possible that the fibronectin-rich environment of the rheumatoid cartilage surface effectively traps pannus cells expressing high levels of these molecules. The VLA-5 molecule was found in a pericellular and interterritorial matrix distribution in the present study, strongly suggesting that a receptor-ligand interaction between VLA-5 and cartilage matrix may occur at the early stage of pannus formation. Furthermore, an increase in beta 1 integrin may be necessary for the growth of the pannus and also for the upregulation of the VLA molecules, leading secondarily to increased attachment.
AuthorsH Ishikawa, S Hirata, Y Andoh, H Kubo, N Nakagawa, Y Nishibayashi, K Mizuno
JournalRheumatology international (Rheumatol Int) Vol. 16 Issue 2 Pg. 53-60 ( 1996) ISSN: 0172-8172 [Print] Germany
PMID8853226 (Publication Type: Journal Article)
Chemical References
  • Antibodies, Monoclonal
  • Integrins
  • Intercellular Adhesion Molecule-1
Topics
  • Aged
  • Aged, 80 and over
  • Antibodies, Monoclonal
  • Arthritis, Rheumatoid (metabolism, pathology)
  • Cartilage, Articular (metabolism, ultrastructure)
  • Female
  • Fibroblasts (metabolism, ultrastructure)
  • Humans
  • Immunohistochemistry
  • Integrins (metabolism)
  • Intercellular Adhesion Molecule-1 (metabolism)
  • Macrophages (metabolism, ultrastructure)
  • Male
  • Microscopy, Immunoelectron
  • Middle Aged
  • Synovial Membrane (metabolism, ultrastructure)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: