Concanamycin B, a vacuolar H(+)-ATPase specific inhibitor suppresses bone resorption in vitro.

Abstract

Effects of concanamycin B, a specific inhibitor of the vacuolar type H(+)-ATPase (V-ATPase), on the stimulation of bone resorption induced by parathyroid hormone (PTH) were examined in vitro. Concanamycin B was found to inhibit PTH-stimulated osteoclastic pit formation and to suppress the acidification of vacuolar organelles by V-ATPase in the osteoclasts. PTH-stimulated 45Ca release from prelabelled chick embryonic calvariae was also inhibited by concanamycin B in a dose-dependent manner. These results suggest that osteoclastic acidification of lacunae by V-ATPase plays an essential role in mineral dissolution and degradation of the organic matrix during bone resorption.

• Authors: J T Woo, Y Ohba, K Tagami, K Sumitani, K Yamaguchi, T Tsuji
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 19 Issue 2 Pg. 297-9 (Feb 1996) ISSN: 0918-6158 [Print] Japan
• PMID: 8850326 (Publication Type: Journal Article)

Chemical References

• Anti-Bacterial Agents
• Macrolides
• Parathyroid Hormone
• concanamycin B
• Proton-Translocating ATPases
• Calcium

Topics

• Animals
• Anti-Bacterial Agents (pharmacology)
• Bone Resorption (prevention & control)
• Calcium (metabolism)
• Humans
• Macrolides
• Parathyroid Hormone (pharmacology)
• Proton-Translocating ATPases (antagonists & inhibitors, physiology)
• Rats
• Rats, Sprague-Dawley
• Vacuoles (enzymology)