Effects on complement activation and cytokine (TNF-alpha and IL-8) release of infusion of anti-TNF-antibodies or a xanthine derivative (HWA 138) in septic baboons.

Abstract

BACKGROUND:

Sepsis and septic shock lead to activation of the complement cascade and to the release of pro-inflammatory cytokines.

METHODS:

The effects of E coli infusion and of infusion of anti-TNF antibodies and a xanthine derivative (HWA 138) on complement activation and cytokine release was evaluated in 17 baboons. All animals received 5 x 10(8) live bacteria per kg body weight. Five animals received only bacteria, five received in addition 0.5 mg per kg body weight of anti-TNF-antibody, and seven received an infusion of 6 mg per kg body weight of HWA 138 in addition to the bacteria.

RESULTS:

In baboons receiving 5 x 10(8) live E coli per kg body weight increased plasma levels of TCC, TNF-alpha and IL-8 were found. The release of TNF-alpha was lower in the group receiving HWA 138 at 2 h after the infusion. In baboons receiving an infusion of anti-TNF antibody the concentration of IL-8 was lower at 2 and 4 h than in animals receiving just E coli or HWA 138.

CONCLUSION:

Infusion of anti-TNF-antibody before E coli infusion will decrease the formation of IL-8. Infusion of HWA 138 before the E coli infusion will also inhibit the formation of TNF-alpha.

• Authors: A Bengtsson, H Redl, G Schlag, T E Mollnes, K Högåsen
• Journal: Acta anaesthesiologica Scandinavica (Acta Anaesthesiol Scand) Vol. 40 Issue 2 Pg. 244-9 (Feb 1996) ISSN: 0001-5172 [Print] England
• PMID: 8848926 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• Complement Membrane Attack Complex
• Interleukin-8
• Tumor Necrosis Factor-alpha
• Xanthines
• 1-(5-hydroxy-5-methylhexyl)-3-methylxanthine
• Pentoxifylline

Topics

• Animals
• Antibodies (administration & dosage)
• Complement Activation
• Complement Membrane Attack Complex (metabolism)
• Escherichia coli Infections (immunology, physiopathology)
• Hemodynamics
• Interleukin-8 (biosynthesis)
• Male
• Papio
• Pentoxifylline (analogs & derivatives, pharmacology)
• Sepsis (immunology, physiopathology)
• Tumor Necrosis Factor-alpha (biosynthesis, immunology)
• Xanthines (pharmacology)