HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Characteristics of the antitumor activity of M-16 and M-18, major metabolites of a new mitomycin C derivative KW-2149, in mice.

Abstract
The cell growth inhibitory activity, antitumor activity and toxicity of M-16 and M-18, the major metabolites of a new mitomycin C (MMC) derivative KW-2149, in mouse and human were compared with those of KW-2149 or MMC in vitro and in vivo. The growth inhibitory activity of M-18, a symmetrical disulfide dimer, against human uterine cervix carcinoma HeLa S3 cells was almost equivalent to that of KW-2149 and their IC50 values were about 10-fold smaller than that of MMC. The activity of M-16, a methyl sulfide form, was almost equivalent to that of MMC. The cell-killing activity of MMC and M-16 was augmented in the hypoxic condition, whereas that of KW-2149 and M-18 was reduced. M-16 also exhibited almost equivalent activities to MMC in vivo in terms of many biological profiles, i.e., antitumor activity against murine P388 leukemia, ascitic or solid B16 melanoma or human lung carcinoma xenograft L-27, and bone marrow toxicity in mice. These in vitro and in vivo results indicate that the antitumor activity and toxicity of KW-2149 might not be mediated by M-16 in mice. On the other hand, M-18 exhibited almost equivalent activities to KW-2149 in these regards, suggesting the involvement of M-18 in the biological activities of KW-2149. However, the small values of the area under the curve of M-18 in mice make this unlikely. Thus the biological activities of KW-2149 in mice are not explained by the M-16 or M-18 concentration in plasma and are postulated to be manifested by KW-2149 itself.
AuthorsT Ashizawa, A Okamoto, M Okabe, S Kobayashi, H Arai, H Saito, M Kasai, K Gomi
JournalAnti-cancer drugs (Anticancer Drugs) Vol. 6 Issue 6 Pg. 763-70 (Dec 1995) ISSN: 0959-4973 [Print] England
PMID8845489 (Publication Type: Journal Article)
Chemical References
  • Antineoplastic Agents
  • Mitomycins
  • KW 2149
  • Mitomycin
Topics
  • Animals
  • Antineoplastic Agents (pharmacokinetics, pharmacology, toxicity)
  • Biotransformation
  • Breast Neoplasms (drug therapy)
  • Cell Survival (drug effects)
  • HeLa Cells
  • Humans
  • Lethal Dose 50
  • Leukemia P388 (drug therapy)
  • Male
  • Melanoma, Experimental (drug therapy)
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Mice, Inbred Strains
  • Mitomycin (pharmacokinetics, pharmacology, toxicity)
  • Mitomycins
  • Rats
  • Sarcoma 180 (drug therapy)
  • Tumor Cells, Cultured

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: