Abstract |
Colonization factor antigens (CFAs) mediate attachment of enterotoxigenic Escherichia coli (ETEC) to the intestinal mucosa and induce protective immunity against ETEC diarrhea. ETEC strains (n = 111) isolated from North Indian children from 1985 to 1989 were examined for CFAs and putative colonization factors (PCFs). CFA/IV was the most common factor (26%), followed by coli surface antigen 17 (CS17) (19%), CFA/I (14%), PCFO166 (7%), and CFA/II (5%), while 24% of the isolates were negative for CFAs and PCFs. Among the strains producing heat-stable and heat-labile toxin (ST+LT+ strains), the STaI gene was strongly associated with the absence of known CFAs and PCFs, making the STaI+LT+ isolates an interesting target for the identification of previously undescribed factors. Repetitive sequence--based polymerase chain reaction revealed that the CS17+ strains, although clonally related, represented endemically circulating strains with a diversity greater than that of the CFA/I+ strains, which showed a substantial clonal clustering.
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Authors | H Sommerfelt, H Steinsland, H M Grewal, G I Viboud, N Bhandari, W Gaastra, A M Svennerholm, M K Bhan |
Journal | The Journal of infectious diseases
(J Infect Dis)
Vol. 174
Issue 4
Pg. 768-76
(Oct 1996)
ISSN: 0022-1899 [Print] United States |
PMID | 8843215
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Bacterial Proteins
- Bacterial Toxins
- Bacterial Vaccines
- Enterotoxins
- Escherichia coli Proteins
- colonization factor antigens
- heat stable toxin (E coli)
- Fimbriae Proteins
- heat-labile enterotoxin, E coli
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Topics |
- Bacterial Proteins
(genetics)
- Bacterial Toxins
(analysis, genetics)
- Bacterial Vaccines
(immunology)
- Child, Preschool
- Enterotoxins
(analysis, genetics)
- Escherichia coli
(pathogenicity)
- Escherichia coli Proteins
- Fimbriae Proteins
- Genotype
- Humans
- Infant
- Polymerase Chain Reaction
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