Abstract |
1. The antithrombotic effect of aurintricarboxylic acid (ATA) which inhibits binding of von Willebrand factor (vWF) to platelet glycoprotein lb (GPlb) receptor was evaluated in photochemically-induced thrombosis models in the femoral artery of rats and guinea-pigs. 2. ATA at a dose of 10 mg kg-1 significantly prolonged the time required for thrombotic occlusion of the artery in rats. The antithrombotic efficacy was associated with a significant inhibition of platelet retention and ex vivo botrocetin-induced platelet aggregation. 3. On the other hand, in guinea-pigs, ATA at the same dose inhibited the platelet retention and the platelet aggregation, but did not prevent thromboocclusion. 4. ATA inhibited aggregation of washed platelets from rats or guinea-pigs in response to botrocetin and thrombin in a dose-dependent manner (1-30 microM), and to the same extent. 5. ATA moderately increased activated partial thromboplastin time and bleeding time in both species. 6. These results indicate that vWF may play a role in the development of occlusive arterial thrombosis in the rat, but not in the guinea-pig. 7. The antithrombotic activity of ATA may partly arise from its inhibitory effect on thrombin, in addition to that on the vWF-GPlb pathway
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Authors | Y Takiguchi, M Shimazawa, M Nakashima |
Journal | British journal of pharmacology
(Br J Pharmacol)
Vol. 118
Issue 7
Pg. 1633-8
(Aug 1996)
ISSN: 0007-1188 [Print] England |
PMID | 8842425
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Fibrinolytic Agents
- von Willebrand Factor
- Aurintricarboxylic Acid
- Collagen
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Topics |
- Animals
- Aurintricarboxylic Acid
(therapeutic use)
- Bleeding Time
- Blood Coagulation
(drug effects)
- Collagen
(pharmacology)
- Femoral Artery
(pathology)
- Fibrinolytic Agents
(therapeutic use)
- Guinea Pigs
- Male
- Partial Thromboplastin Time
- Platelet Aggregation
(drug effects)
- Rats
- Rats, Wistar
- Thrombosis
(blood, drug therapy, pathology)
- von Willebrand Factor
(antagonists & inhibitors)
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