Hydroxyindole-O-methyltransferase in Y-79 cells: regulation by serum.

Abstract

Hydroxyindole-O-methyltransferase (HIOMT) catalyzes the last step in the synthesis of melatonin. In the present study, the regulation of HIOMT expression was examined in the human Y-79 retinoblastoma cell line. Cells were grown in suspension culture using medium supplemented with 10% fetal calf serum (FCS). HIOMT activity and mRNA were strongly reduced when FCS was substituted with 0.1% bovine serum albumin (BSA), and were restored by addition of FCS. The effect of FCS on HIOMT expression was relatively selective, because the abundance of mRNA encoding actin, G3PDH or interphotoreceptor retinoid-binding protein did not change following serum deprivation. However, S-antigen (arrestin) mRNA was regulated by serum coordinately with HIOMT mRNA, suggesting that S-antigen expression is also controlled by a serum factor. The effect of serum on HIOMT expression was not duplicated by treatment with a series of known differentiating factors, nor was it reduced by dialysis or stripping procedures which remove steroids, growth factors and thyroid hormones.

• Authors: M Bernard, P Voisin, D C Klein
• Journal: Brain research (Brain Res) Vol. 727 Issue 1-2 Pg. 118-24 (Jul 15 1996) ISSN: 0006-8993 [Print] Netherlands
• PMID: 8842389 (Publication Type: Journal Article)

Chemical References

• Culture Media
• Culture Media, Serum-Free
• DNA Primers
• RNA, Messenger
• Acetylserotonin O-Methyltransferase

Topics

• Acetylserotonin O-Methyltransferase (biosynthesis, isolation & purification, metabolism)
• Animals
• Base Sequence
• Blood
• Cattle
• Cell Line
• Culture Media
• Culture Media, Serum-Free
• DNA Primers
• Eye Neoplasms
• Humans
• Kinetics
• Molecular Sequence Data
• Polymerase Chain Reaction
• RNA, Messenger (biosynthesis)
• Retinoblastoma
• Transcription, Genetic
• Tumor Cells, Cultured