Antineutrophil cytoplasmic antibodies in reactive arthritis.

Abstract

OBJECTIVE:

To study the occurrence of antineutrophil cytoplasmic antibodies (ANCA) in reactive arthritis (ReA).

METHODS:

Sera from 22 patients with ReA were analyzed by ELISA for the presence of autoantibodies (IgG and IgA) against a proteinase-3 containing azurophilic granule extract ("alpha-antigen") from human polymorphonuclear leukocytes, myeloperoxidase (MPO), and lactoferrin (Lf), respectively. Rheumatoid factor (RF), antinuclear antibodies (ANA), and HLA-B27 were also tested. Erythrocyte sedimentation rate and serum levels of C-reactive protein were used to assess disease activity. The patients were divided into acute or chronic (> 1 year) disease.

RESULTS:

12/22 patients (55%) had IgG ANCA (7 had MPO ANCA, 8 had Lf ANCA, and 4 had alpha-ANCA). Eight patients (36%) had IgA ANCA. One serum was positive only for IgA ANCA. 18/21 patients (86%) were HLA-B27 positive, and none had RF or ANA. The triggering infection was Chlamydia trachomatis in 6 cases. Campylobacter jejuni in 6, Yersinia enterocolitica in 4. In 6 patients the causative microorganism could not be determined. ANCA was more prevalent in chronic disease (6/7, 82%) compared to acute (7/15, 47%). No obvious correlation was seen between ANCA and disease activity.

CONCLUSION:

ANCA, predominantly those reacting with Lf and/or MPO preparations, are common in ReA.

• Authors: H Locht, E Peen, T Skogh
• Journal: The Journal of rheumatology (J Rheumatol) Vol. 22 Issue 12 Pg. 2304-6 (Dec 1995) ISSN: 0315-162X [Print] Canada
• PMID: 8835566 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Autoantibodies
• Immunoglobulin A
• Immunoglobulin G
• Immunoglobulin Isotypes
• PHB2 protein, human
• Prohibitins
• Rheumatoid Factor
• Lactoferrin

Topics

• Adult
• Arthritis, Reactive (immunology, microbiology)
• Autoantibodies (analysis, blood)
• Bacterial Infections (diagnosis)
• Campylobacter (isolation & purification)
• Chlamydia (isolation & purification)
• Chronic Disease
• Cytoplasm (immunology)
• Enzyme-Linked Immunosorbent Assay
• Female
• Humans
• Immunoglobulin A (analysis)
• Immunoglobulin G (analysis)
• Immunoglobulin Isotypes (analysis)
• Lactoferrin (immunology)
• Male
• Middle Aged
• Neutrophils (immunology)
• Precipitating Factors
• Prohibitins
• Rheumatoid Factor (analysis)
• Serologic Tests
• Yersinia (isolation & purification)