Molecular cloning and chromosomal localization of the human cyclin C (CCNC) and cyclin E (CCNE) genes: deletion of the CCNC gene in human tumors.

Abstract

The human Gi-phase cyclins are important regulators of cell cycle progression that interact with various cyclin-dependent kinases and facilitate entry into S-phase. We have confirmed the localization of the human cyclin C (CCNC) gene to chromosome 6q21 and of human cyclin E (CCNE) to 19q12. The CCNC gene structure was also determined, and we have shown that it is deleted in a subset of acute lymphoblastic leukemias, including a patient sample containing a t(2;6)(p21;q15), with no apparent cytogenetic deletion. Single-strand conformational polymorphism analysis of the remaining CCNC allele from patients with a deletion of one allele established that there were no further mutations within the exons or the flanking intronic sequences. These results suggest either that haploinsufficiency of the cyclin C protein is sufficient to promote tumorigenesis or that the important tumor suppressor gene is linked to the CCNC locus.

• Authors: H Li, J M Lahti, M Valentine, M Saito, S I Reed, A T Look, V J Kidd
• Journal: Genomics (Genomics) Vol. 32 Issue 2 Pg. 253-9 (Mar 01 1996) ISSN: 0888-7543 [Print] United States
• PMID: 8833152 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• CCNC protein, human
• Cyclin C
• Cyclins

Topics

• Chromosome Mapping
• Chromosomes, Human, Pair 19
• Chromosomes, Human, Pair 6
• Cloning, Molecular
• Cyclin C
• Cyclins (genetics)
• Gene Deletion
• Humans
• Molecular Sequence Data
• Precursor Cell Lymphoblastic Leukemia-Lymphoma (genetics)