Pretargeted tumour imaging with streptavidin immunoconjugates of monoclonal antibody CC49 and 111In-DTPA-biocytin.

Abstract

Pretargeted tumour imaging was performed in nude mice bearing subcutaneous LS174T human colon cancer xenografts with streptavidin-CC49 monoclonal antibody and 111In-DTPA-biocytin. Mice were administered 250 micrograms of streptavidin-CC49, followed 6 or 9 days later by 40 ng (250 microCi) of 111In-DTPA-biocytin. Tumors were visualized at 24 h postinjection of 111In-DTPA-biocytin. Tumour uptake was 0.9-2.5% injected dose/g with tumour/nontarget ratios from 2:1 to 37:1 (except for kidney, which was 0.5-3:1). Tumour uptake in mice pretargeted with streptavidin or streptavidin-conjugated nonspecific normal mouse IgG was < 0.1% i.d./g.

• Authors: T M Alvarez-Diez, J Polihronis, R M Reilly
• Journal: Nuclear medicine and biology (Nucl Med Biol) Vol. 23 Issue 4 Pg. 459-66 (May 1996) ISSN: 0969-8051 [Print] United States
• PMID: 8832700 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antigens, Neoplasm
• Bacterial Proteins
• Glycoproteins
• Immunoconjugates
• Indium Radioisotopes
• alpha,omega-(bis)DTPA-biocytin
• tumor-associated antigen 72
• Pentetic Acid
• Streptavidin
• Lysine

Topics

• Animals
• Antibodies, Monoclonal (pharmacokinetics)
• Antigens, Neoplasm (immunology, metabolism)
• Bacterial Proteins (pharmacokinetics)
• Colonic Neoplasms (diagnostic imaging, metabolism)
• Female
• Glycoproteins (immunology, metabolism)
• Humans
• Immunoconjugates (pharmacokinetics)
• Indium Radioisotopes (pharmacokinetics)
• Lysine (analogs & derivatives, pharmacokinetics)
• Mice
• Mice, Nude
• Neoplasm Transplantation
• Pentetic Acid (analogs & derivatives, pharmacokinetics)
• Radionuclide Imaging
• Streptavidin
• Tissue Distribution
• Transplantation, Heterologous