Chimeric anti-tenascin antibody 81C6: increased tumor localization compared with its murine parent.

Abstract

When labeled using the Iodogen method, a chimeric antibody composed of the human IgG2 constant region and the variable regions of murine anti-tenascin 81C6 exhibited superior uptake in human glioma xenografts compared with its murine parent. In the current study, three paired-label experiments were performed in athymic mice with subcutaneous D-54 MG human glioma xenografts to evaluate further the properties of radioiodinated chimeric 81C6. These studies demonstrated that (a) the enhanced tumor uptake of chimeric 81C6 is specific; (b) when labeling was performed using N-succinimidyl 3-iodobenzoate, chimeric 81C6 again showed preferential accumulation in tumor compared with murine 81C6; and (c) the tumor uptake advantage observed previously with murine 81C6 for N-succinimidyl 3-iodobenzoate compared with Iodogen labeling did not occur with chimeric 81C6.

• Authors: M R Zalutsky, G E Archer, P K Garg, S K Batra, D D Bigner
• Journal: Nuclear medicine and biology (Nucl Med Biol) Vol. 23 Issue 4 Pg. 449-58 (May 1996) ISSN: 0969-8051 [Print] United States
• PMID: 8832699 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Immunoglobulin G
• Immunotoxins
• Iodine Radioisotopes
• Recombinant Fusion Proteins
• Tenascin

Topics

• Animals
• Antibodies, Monoclonal (pharmacokinetics)
• Brain Neoplasms (metabolism)
• Glioma (metabolism)
• Humans
• Immunoglobulin G (metabolism)
• Immunotoxins (pharmacokinetics)
• Iodine Radioisotopes (pharmacokinetics)
• Isotope Labeling
• Mice
• Mice, Nude
• Neoplasm Transplantation
• Recombinant Fusion Proteins (pharmacokinetics)
• Tenascin (immunology)
• Tissue Distribution
• Transplantation, Heterologous