Geranylgeraniol causes a decrease in levels of calreticulin and tyrosine phosphorylation of a 36-kDa protein prior to the appearance of apoptotic features in HL-60 cells.

Abstract

It was demonstrated recently that geranylgeraniol (GGO) has potent apoptosis-inducing activity in various lines of tumor cells, including HL-60 cells. In the present study, we found that GGO markedly inhibited the expression of a calcium-binding protein, calreticulin, prior to the induction of apoptosis in HL-60 cells. Furthermore, we also observed a significant decrease in the tyrosine phosphorylation of a 36-kDa protein that is a major tyrosine-phosphorylated protein in HL-60 cells. These findings suggest that decreases in levels of calreticulin and in the tyrosine phosphorylation of the 36-kDa protein might be associated with the induction of apoptosis by GGO in HL-60 cells.

• Authors: S Nakajo, M Okamoto, Y Masuda, I Sakai, S Ohsawa, K Nakaya
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 226 Issue 3 Pg. 741-5 (Sep 24 1996) ISSN: 0006-291X [Print] United States
• PMID: 8831684 (Publication Type: Journal Article)

Chemical References

• Calcium-Binding Proteins
• Calreticulin
• Diterpenes
• Molecular Chaperones
• Peptide Fragments
• Phosphoproteins
• RNA, Messenger
• Ribonucleoproteins
• Phosphotyrosine
• geranylgeraniol

Topics

• Amino Acid Sequence
• Apoptosis (drug effects)
• Calcium-Binding Proteins (biosynthesis, chemistry, metabolism)
• Calreticulin
• Diterpenes (pharmacology)
• HL-60 Cells
• Humans
• Kinetics
• Molecular Chaperones (metabolism)
• Molecular Weight
• Peptide Fragments (chemistry, isolation & purification)
• Phosphoproteins (chemistry, isolation & purification, metabolism)
• Phosphorylation
• Phosphotyrosine (metabolism)
• RNA, Messenger (biosynthesis)
• Ribonucleoproteins (biosynthesis, chemistry, metabolism)
• Time Factors
• Transcription, Genetic