Construction of a YAC contig encompassing the Usher syndrome type 1C and familial hyperinsulinism loci on chromosome 11p14-15.1.

Abstract

The Usher syndrome type 1C (USH1C) and familial hyperinsulinism (HI) loci have been assigned to chromosome 11p14-15.1, within the interval D11S419-D11S1310. We have constructed a yeast artificial chromosome (YAC) contig, extending from D11S926 to D11S899, which encompasses the critical regions for both USH1C and HI and spans an estimated genetic distance of approximately 4 cM. A minimal set of six YAC clones constitute the contig, with another 22 YACs confirming the order of sequence-tagged sites (STSs) and position of YACs on the contig. A total of 40 STSs, including 10 new STSs generated from YAC insert-end sequences and inter-Alu PCR products, were used to order the clones within the contig. This physical map provides a resource for identification of gene transcripts associated with USH1C, HI, and other genetic disorders that map to the D11S926-D11S899 interval.

• Authors: R Ayyagari, A Nestorowicz, Y Li, S Chandrasekharappa, C Chinault, P van Tuinen, R J Smith, J F Hejtmancik, M A Permutt
• Journal: Genome research (Genome Res) Vol. 6 Issue 6 Pg. 504-14 (Jun 1996) ISSN: 1088-9051 [Print] United States
• PMID: 8828039 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA Primers
• Genetic Markers

Topics

• Chromosome Mapping
• Chromosomes, Artificial, Yeast (genetics)
• Chromosomes, Human, Pair 11 (genetics)
• Cloning, Molecular
• DNA Primers (chemistry)
• Genetic Diseases, Inborn (genetics)
• Genetic Markers (genetics)
• Humans
• Hyperinsulinism (genetics)
• Sequence Tagged Sites