Cell targeting for gene delivery: use of fusion protein containing the modified human receptor for ecotropic murine leukemia virus.

Abstract

We have previously cloned a human gene (H13) homologous to the murine ecotropic retrovirus (E-MuLV) receptor, which, however, does not confer susceptibility to E-MuLV infection. The extracellular domain 3 (ECD3) of H13 contains amino acid residues critical for E-MuLV binding in that the modified H13 gene (mH13), substituted with amino acids from the actual receptor, has the ability to bind E-MuLV. Here we have expressed a fusion protein consisting of mH13/ECD3 and transforming growth factor-alpha in Escherichia coli and demonstrated its binding activity to both ecotropic AKR virus and the epidermal growth factor receptor expressed on the cell surface. Fusion proteins of mH13/ECD3 and ligands to cell surface molecules might be useful for specific cell targeting in E-MuLV-based gene delivery systems.

• Authors: K Ohno, G D Brown, D Meruelo
• Journal: Biochemical and molecular medicine (Biochem Mol Med) Vol. 56 Issue 2 Pg. 172-5 (Dec 1995) ISSN: 1077-3150 [Print] United States
• PMID: 8825081 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Recombinant
• Receptors, Virus
• Recombinant Fusion Proteins
• Transforming Growth Factor alpha

Topics

• Amino Acid Sequence
• Base Sequence
• Cloning, Molecular
• DNA, Recombinant
• Escherichia coli (genetics)
• Gene Targeting
• Humans
• Leukemia Virus, Murine (physiology)
• Membrane Fusion (genetics, physiology)
• Molecular Sequence Data
• Receptors, Virus (genetics)
• Recombinant Fusion Proteins (genetics)
• Transforming Growth Factor alpha (genetics)
• Tumor Cells, Cultured