Biodistribution of anti-CEA F(ab')2 fragments after intra-arterial and intravenous injection in patients with liver metastases due to colorectal carcinoma.

Abstract

The biodistribution of simultaneous intra-arterial and intravenous injections of a radiolabelled anti-CEA MAb F(ab')2 fragment was studied in three patients with liver metastases from colorectal cancer. Identical MAb fragments, labelled with either 125I or 131I, were injected over a period of 30 min into the hepatic artery and into a peripheral vein. After 1 or 2 days, biodistribution was measured in the surgically removed metastases, normal tissue samples and blood. By tissue radioactivity counting, tumour uptake in the range 6.3-9.1% of injected dose per gram was found. Superimposable metastasis-to-blood and metastasis-to-normal liver ratios were obtained for both iodine isotopes in all three patients. The results indicate that the intra-arterial injection of MAb F(ab')2 fragments gives no measurable advantage over more convenient injections into a peripheral vein.

• Authors: F Buchegger, M Gillet, F Doenz, C A Vogel, C Achtari, J P Mach, B Delaloye, A B Delaloye
• Journal: Nuclear medicine communications (Nucl Med Commun) Vol. 17 Issue 6 Pg. 500-3 (Jun 1996) ISSN: 0143-3636 [Print] England
• PMID: 8822748 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Carcinoembryonic Antigen
• Immunoglobulin Fab Fragments
• Iodine Radioisotopes

Topics

• Antibodies, Monoclonal
• Carcinoembryonic Antigen (immunology)
• Colonic Neoplasms (diagnostic imaging, pathology)
• Female
• Humans
• Immunoglobulin Fab Fragments
• Injections, Intra-Arterial
• Injections, Intravenous
• Iodine Radioisotopes (administration & dosage, pharmacokinetics)
• Liver Neoplasms (diagnostic imaging, pathology, secondary, surgery)
• Male
• Middle Aged
• Radioimmunodetection
• Rectal Neoplasms (diagnostic imaging, pathology)
• Sensitivity and Specificity
• Tissue Distribution