Abstract |
Placenta lactogen-I variant ( PL-Iv) is a member of a family of proteins expressed by the rat placenta with characteristics similar to prolactin (PRL). In this report, we present the molecular cloning, chromosomal localization, and heterologous expression of PL-Iv. Nucleotide sequence analysis of the PL-Iv cDNA clone predicted a precursor protein of 223 amino acids, including a 28-amino acid signal sequence. The PL-Iv gene was localized to chromosome 17 of the rat genome, which also carries other members of the PRL gene family. PL-Iv heterologously expressed in Chinese Hamster ovary (CHO) cells exhibited similar immunoreactive and electrophoretic characteristics with PL-Iv produced by the rat placenta. N-terminal sequencing verified the identity and purity of the recombinant PL-Iv species and the site of cleavage of the signal peptide from the mature secreted PL-Iv species. Recombinant PL-Iv was shown to bind to ovarian and liver PRL receptors, stimulate the proliferation of Nb2 lymphoma cells, and activate Jak2. Each of these actions is consistent with PL-Iv utilizing the PRL receptor signal transduction pathway.
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Authors | C B Cohick, G Dai, L Xu, S Deb, T Kamei, G Levan, C Szpirer, J Szpirer, S C Kwok, M J Soares |
Journal | Molecular and cellular endocrinology
(Mol Cell Endocrinol)
Vol. 116
Issue 1
Pg. 49-58
(Jan 15 1996)
ISSN: 0303-7207 [Print] Ireland |
PMID | 8822264
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- DNA, Complementary
- Protein Sorting Signals
- Receptors, Prolactin
- placental lactogen I, rat
- Placental Lactogen
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Topics |
- Animals
- Chromosome Mapping
- Cloning, Molecular
- Cricetinae
- DNA, Complementary
(genetics)
- Female
- Gene Expression
- Genetic Variation
- Placental Lactogen
(genetics, metabolism)
- Pregnancy
- Protein Sorting Signals
(genetics, metabolism)
- Rats
- Receptors, Prolactin
(metabolism)
- Signal Transduction
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