Abstract | BACKGROUND: METHODS: Twelve adult mongrel dogs underwent left lung allotransplantation. The lungs of the donor dogs were flushed with University of Wisconsin solution (50 ml/kg). Six donor dogs were pretreated with U74500A (5 mg/kg intravenously) before preservation (group L, n = 6), whereas those dogs without pretreatment served as controls (group C, n = 6). Allografts were stored in University of Wisconsin solution for 24 hours at 1 degrees C. Left single lung transplantations were performed by means of standard technique. Before reperfusion, recipients in group L received another dose of U74500A. Arterial blood gas analysis and hemodynamic measurements were made by occluding the right pulmonary artery to evaluate the transplanted left lung function at a inspired oxygen fraction of 1.0. Serum lipid peroxide level was measured after 2 hours of reperfusion. RESULTS: Arterial oxygen tension, arterial carbon dioxide tension, and left pulmonary vascular resistance at 6 hours after reperfusion were significantly better in group L than in group C (arterial oxygen tension: 510 +/- 66 and 219 +/- 149 mm Hg; arterial carbon dioxide tension: 47 +/- 16 and 68 +/- 14 mm Hg; left pulmonary vascular resistance: 2412 +/- 826 and 3904 +/- 1251 dyn center dot sec/cm5, group L and group C, respectively). Serum lipid peroxide level was significantly lower in group L (0.25 +/- 0.24 nmol/ml) than in group C (0.92 +/- 0.53). CONCLUSIONS: The administration of U74500A prevented lipid peroxidation and preserved pulmonary allograft function after 24 hours of ischemia.
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Authors | Y Tanoue, S Morita, Y Ochiai, Q W Zhang, M Hisahara, K Miyamoto, T Nishida, Y Kawachi, R Tominaga, H Yasui |
Journal | The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation
(J Heart Lung Transplant)
Vol. 15
Issue 1 Pt 1
Pg. 43-50
(Jan 1996)
ISSN: 1053-2498 [Print] United States |
PMID | 8820082
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Antioxidants
- Pregnatrienes
- U 74500A
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Topics |
- Animals
- Antioxidants
(pharmacology)
- Dogs
- Lipid Peroxidation
(drug effects)
- Lung
(drug effects, physiology)
- Lung Transplantation
(methods, physiology)
- Organ Preservation
(methods)
- Perfusion
(methods)
- Pregnatrienes
(pharmacology)
- Reperfusion
(methods)
- Time Factors
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