Abstract | OBJECTIVE: METHODS: Isolated buffer-perfused rat hearts (100 cm H2O pressure (= 73.5 mmHg), 37 degrees C) were used. After a stabilization period the perfusion of 8 rats (group 1) was stopped and the hearts arrested with 5 ml CS (100 cm H2O, 12 degrees C). Equal amounts of cardioplegic solution were then delivered every 20 minutes for the entire 3 1/2 hour hypothermic ischemic period. Following ischemia the hearts were reperfused for 60 minutes. In group 2 (n = 8) 1 ml 10(-2) mmol Papaverine was given into the aortic root just prior to the first cardioplegic solution infusion in order to induce coronary vasodilation. The procedure was identical in the two groups during ischemia and reperfusion. RESULTS: During the ischemic period coronary resistance increased in group 2. During reperfusion group 2 had lower coronary flow (P = 0.001), left ventricle developed pressure (P = 0.002) and a higher creatine kinase release (P = 0.003) than group 1 hearts. Group 2 also had a lower adenosine-triphosphate (6.51 +/- 0.40 mumol.g-1 and 14.03 +/- 0.59 mumol.g-1, respectively, P = 0.011), creatine phosphate (24.70 +/- 1.02 mumol.g-1 and 36.50 +/- 1.31 mumol.g-1, respectively, P = 0.020) and a larger fall in dry/wet-weight ratio (1.7 +/- 0.4 and 0.8 +/- 0.5, respectively, P = 0.043). CONCLUSIONS:
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Authors | O Irtun, D G Sørlie |
Journal | European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery
(Eur J Cardiothorac Surg)
Vol. 10
Issue 6
Pg. 456-60
( 1996)
ISSN: 1010-7940 [Print] Germany |
PMID | 8817143
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Animals
- Cardioplegic Solutions
(administration & dosage)
- Coronary Circulation
(physiology)
- Hemodynamics
(physiology)
- Homeostasis
(physiology)
- Male
- Myocardial Ischemia
(physiopathology)
- Myocardial Reperfusion Injury
(physiopathology)
- Rats
- Rats, Wistar
- Vascular Resistance
(physiology)
- Vasodilation
(physiology)
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