Abstract |
4-beta-phorbol 12-myristate 13-acetate (PMA), when administered topically to mouse ear, induces a pronounced inflammatory response mediated by protein kinase C (PKC). Activation of PKC is implicated in the pathogenesis of inflammation, with phospholipase A2-dependent arachidonic acid release and eicosanoid production. We have investigated the effects of hydroxyachillin, a sesquiterpene lactone from Tanacetum microphyllum DC., on mouse ear oedema induced by PMA. The effects of this compound on swelling and other inflammatory parameters are described. Hydroxyachillin significantly (p < or = 0.01) inhibited ear swelling in a dose-dependent manner, and was as effective as the reference drugs. The PMA-induced vascular permeability was significantly (p < or = 0.05) reduced by hydroxyachillin at the highest dose (3 mg/ear). Histologically, the signs of inflammation were greatly reduced in the hydroxyachillin-treated ear lesions. These data suggest that hydroxyachillin is an effective anti-inflammatory agent in this model, and that the inhibition of PKC may be one of the mechanisms of hydroxyachillin's effect.
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Authors | A M Silván, M J Abad, P Bermejo, A Villar |
Journal | Inflammation research : official journal of the European Histamine Research Society ... [et al.]
(Inflamm Res)
Vol. 45
Issue 6
Pg. 289-92
(Jun 1996)
ISSN: 1023-3830 [Print] Switzerland |
PMID | 8814460
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- Carcinogens
- Eicosanoids
- Lactones
- Sesquiterpenes
- hydroxyachillin
- Arachidonic Acid
- Protein Kinase C
- Phospholipases A
- Phospholipases A2
- Tetradecanoylphorbol Acetate
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Topics |
- Administration, Topical
- Animals
- Anti-Inflammatory Agents, Non-Steroidal
(administration & dosage, pharmacology, therapeutic use)
- Arachidonic Acid
(metabolism)
- Capillary Permeability
(drug effects)
- Carcinogens
(administration & dosage, toxicity)
- Dose-Response Relationship, Drug
- Ear
(pathology)
- Edema
(chemically induced, drug therapy)
- Eicosanoids
(metabolism)
- Enzyme Activation
(drug effects)
- Female
- Lactones
(administration & dosage, pharmacology, therapeutic use)
- Lethal Dose 50
- Mice
- Phospholipases A
(metabolism)
- Phospholipases A2
- Protein Kinase C
(metabolism)
- Reference Standards
- Regression Analysis
- Sesquiterpenes
(administration & dosage, pharmacology, therapeutic use)
- Tetradecanoylphorbol Acetate
(administration & dosage, toxicity)
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