Abstract |
In whole-cell recordings from CA1 neurons in slices from rats, the mGLUR agonist (+/-)-1-aminocyclopentane-trans-1,3-dicarboxylic acid (ACPD; 10 microM) had a depolarizing action on most cells, associated with an increase in input resistance and suppression of afterhyperpolarizations. Under voltage-clamp, there were corresponding changes in membrane current and conductance; in the presence of ACPD, the slow voltage-dependent outward current recorded at approximately -25 mV was smaller and was more clearly depressed by hypoxia. Neither ACPD nor mGLUR antagonists, L(+)-2-amino-3-phosphonoproprionic acid (L-AP3; 1 mM) and (+)-alpha-methyl-4-carboxyphenyl- glycine (MCPG; 0.5 mM), reduced the hyperpolarization or outward current (or the associated changes in input resistance or conductance) induced by 2 min of hypoxia. Early inward currents, corresponding to the early, transient depolarizing effect of hypoxia, wer also not significantly depressed by either MCPG or L-AP3. The hypoxic responses of CA1 neurons in slices are therefore unlikely to be caused mainly be glutamate release and activation of mGLURs.
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Authors | G Erdemli, Krnjević |
Journal | Brain research
(Brain Res)
Vol. 723
Issue 1-2
Pg. 1-7
(Jun 03 1996)
ISSN: 0006-8993 [Print] Netherlands |
PMID | 8813376
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Receptors, Metabotropic Glutamate
- Cycloleucine
- 1-amino-1,3-dicarboxycyclopentane
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Topics |
- Animals
- Cycloleucine
(analogs & derivatives, pharmacology)
- Hippocampus
(drug effects)
- Hypoxia
(physiopathology)
- Male
- Membrane Potentials
(drug effects)
- Patch-Clamp Techniques
- Rats
- Rats, Sprague-Dawley
- Receptors, Metabotropic Glutamate
(drug effects)
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