Abstract |
Myelopeptide-2 (MP-2) Leu-Val-Val-Tyr-Pro-Trp originally isolated from the supernatant of porcine bone marrow cell culture was examined for its capacity to restore the mitogen responsiveness of human T lymphocytes inhibited by conditioned media from HL-60 leukemia cells (HL-60 CM). MP-2 added to phytohemagglutinin (PHA)-stimulated T lymphocytes together with HL-60 CM abolished the suppression of T-lymphocyte proliferative response in a dose-dependent manner. Another bone marrow hexapeptide Phe-Leu- Gly-Phe-Pro-Thr, MP-1, did not display this action in that experimental system. MP-2 was also effective being added after T-lymphocyte exposure to HL-60 CM which suggests its recovery but not protective effect on T-lymphocytes treated with tumor cell products. Flow cytometry analysis revealed HL-60 CM influence on the expression of CD3 and CD4 T-cell surface antigens. It decreased the content of CD3- and CD4-positive cells and induced the appearance of T lymphocytes with reduced density of CD3 and CD4 antigens. MP-2 was able to restore the T-cell phenotype altered by HL-60 CM. MP-2 seems to be promising in anti- tumor therapy.
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Authors | L A Strelkov, A A Mikhailova, A M Sapozhnikov, L A Fonina, R V Petrov |
Journal | Immunology letters
(Immunol Lett)
Vol. 50
Issue 3
Pg. 143-7
(May 1996)
ISSN: 0165-2478 [Print] Netherlands |
PMID | 8803611
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CD3 Complex
- CD4 Antigens
- CD8 Antigens
- Mitogens
- Oligopeptides
- Peptides
- Phytohemagglutinins
- myelopeptides
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Topics |
- Bone Marrow
(immunology)
- Bone Marrow Cells
- CD3 Complex
(metabolism)
- CD4 Antigens
(metabolism)
- CD8 Antigens
(physiology)
- Flow Cytometry
- HL-60 Cells
- Humans
- Immunity, Cellular
- Lymphocyte Activation
(drug effects)
- Mitogens
(pharmacology)
- Oligopeptides
- Peptides
(pharmacology)
- Phytohemagglutinins
(pharmacology)
- T-Lymphocytes
(immunology)
- Up-Regulation
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