Abstract | BACKGROUND AND PURPOSE: In this study we investigated the effects of a novel compound, MS-153 ([R]-[-]-5-methyl-1-nicotinoyl-2-pyrazoline), on elevated brain glutamate concentrations and cerebral infarct volume induced by middle cerebral artery (MCA) occlusion in the rat. METHODS: The rat MCA was occluded by a thrombus induced by a photochemical reaction between green light and the photosensitizer dye rose bengal, which causes endothelial injury followed by formation of a platelet- and fibrin-rich thrombus at the site of photochemical reaction; this method is routinely used in our laboratory to produce arterial occlusion in experimental animals. Extracellular glutamate concentration at the ischemic border zone was determined by a microdialysis technique. The size of cerebral infarction was measured by a histochemical technique 24 hours after MCA occlusion. MS-153 was administered at various doses as a continuous infusion for 24 hours, beginning 0 to 2 hours after MCA occlusion. RESULTS: At the ischemic border zone, the concentration of glutamate in the extracellular fluid increased by 40-fold after ischemia. At 3.13 mg/kg per hour, MS-153 reduced glutamate concentration (P < .05) and also the size of ischemic cerebral infarction (P < .05). Furthermore, the glutamate uptake inhibitor DL-threo-beta-hydroxyaspartate reversed the effect of MS-153 on glutamate concentration. CONCLUSIONS:
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Authors | K Umemura, T Gemba, A Mizuno, M Nakashima |
Journal | Stroke
(Stroke)
Vol. 27
Issue 9
Pg. 1624-8
(Sep 1996)
ISSN: 0039-2499 [Print] United States |
PMID | 8784139
(Publication Type: Journal Article)
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Chemical References |
- 5-methyl-1-nicotinoyl-2-pyrazoline
- Nicotinic Acids
- Glutamine
- Glutamic Acid
- Glycine
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Topics |
- Animals
- Arterial Occlusive Diseases
(metabolism, pathology)
- Brain
(drug effects, metabolism)
- Cerebral Arteries
- Cerebral Infarction
(pathology)
- Glutamic Acid
(metabolism)
- Glutamine
(metabolism)
- Glycine
(metabolism)
- Male
- Nicotinic Acids
(pharmacology)
- Osmolar Concentration
- Rats
- Rats, Wistar
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