Sulfated pentagalloyl glucose (Y-ART-3) inhibits HIV replication and cytopathic effects in vitro, and reduces HIV infection in hu-PBL-SCID mice.

Abstract

To evaluate the efficacy of Y-ART-3 as an antiviral drug for HIV infections, its anti-HIV activity was assessed in vitro in cell culture systems and in vivo in hu-PBL-SCID mice. The results indicated that Y-ART-3 invariably inhibited not only HIV-1, but also HIV-2 and SIV strains. Its mechanism of action is ascribed to inhibition of viral adsorption to CD4-positive cells. In an in vivo study, human Ig- and CD4-positive cells were detected at similar levels in Y-ART-3-treated hu-PBL-SCID mice that were infected with HIV, and in PBS-treated control hu-PBL SCID mice that were not infected with HIV. If HIV positivity was calculated using the number of tests in which HIV was detected (i.e. PCR, and p24 from co-cultures of spleen and peritoneal wash cells), a significant effect of Y-ART-3 at a dose of 4 mg/kg was noted. Therefore, Y-ART-3 may be considered to be a potent and effective anti-HIV compound.

• Authors: H Nakashima, K Ichiyama, F Hirayama, K Uchino, M Ito, T Saitoh, M Ueki, N Yamamoto, H Ogawara
• Journal: Antiviral research (Antiviral Res) Vol. 30 Issue 2-3 Pg. 95-108 (May 1996) ISSN: 0166-3542 [Print] Netherlands
• PMID: 8783802 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antiviral Agents
• HIV Core Protein p24
• Hydrolyzable Tannins
• Tannins
• Y-ART 3
• pentagalloylglucose
• Gallic Acid
• Glucose

Topics

• Animals
• Antiviral Agents (immunology, pharmacology)
• Cell Line
• Cytopathogenic Effect, Viral
• Gallic Acid (analogs & derivatives, chemistry, pharmacology)
• Giant Cells
• Glucose (analogs & derivatives, chemistry, pharmacology)
• HIV Core Protein p24 (metabolism)
• HIV-1 (drug effects, metabolism, physiology)
• HIV-2 (drug effects, physiology)
• Humans
• Hydrolyzable Tannins
• Mice
• Mice, SCID
• Simian Immunodeficiency Virus (drug effects, physiology)
• Tannins (immunology, pharmacology)
• Tumor Cells, Cultured
• Virus Replication