Effect of MDP-Lys(L18) as a mucosal immunoadjuvant on protection of mucosal infections by Sendai virus and rotavirus.

Abstract

To examine the effect of MDP-Lys(L18), a derivative of muramyl dipeptide (MDP), as a mucosal immunoadjuvant, we investigated its activity to augment host resistance against mucosal infections by Sendai virus and rotavirus in mice. In an experimental infection model using suckling mice (10-day-old) inoculated perorally (p.o.) with 1.5 x 10(6) p.f.u. mouse-1 of rotavirus strain SA11, intrarectal (i.r.) as well as p.o. administration of MDP-Lys(L18) (50 micrograms mouse-1) prior to virus infection markedly reduced rotavirus-induced diarrhea. Furthermore, when MDP-Lys(L18) was administered p.o. (1 mg mouse-1), i.r. (300 micrograms mouse-1) or intranasally (i.n., 100 micrograms mouse-1) various days before Sendai virus infection (2.6 x 10(4) HAD mouse-1), all the mucosal administration of MDP-Lys(L18) significantly protected a lethal infection of Sendai virus, showing a dose-dependent manner. However, the efficacy of MDP-Lys(L18) to induce the prophylactic activity against the viruses somewhat varied according to the administration route and timing. In time course analysis of virus isolation in vivo, the mice administered with MDP-Lys(L18) exhibited a significant reduction of both viruses in the lungs for Sendai virus and in the bowels for rotavirus. These results suggest that MDP-Lys(L18) is a potent mucosal immunoadjuvant to enhance nonspecific host resistance against two mucosal infectious viruses, Sendai virus and rotavirus.

• Authors: A Fukushima, Y C Yoo, K Yoshimatsu, K Matsuzawa, M Tamura, S Tono-oka, K Taniguchi, S Urasawa, J Arikawa, I Azuma
• Journal: Vaccine (Vaccine) Vol. 14 Issue 6 Pg. 485-91 (Apr 1996) ISSN: 0264-410X [Print] Netherlands
• PMID: 8782344 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Acetylmuramyl-Alanyl-Isoglutamine
• romurtide

Topics

• Acetylmuramyl-Alanyl-Isoglutamine (administration & dosage, analogs & derivatives, immunology, pharmacology)
• Adjuvants, Immunologic (administration & dosage, pharmacology)
• Administration, Intranasal
• Administration, Oral
• Administration, Rectal
• Animals
• Dose-Response Relationship, Immunologic
• Female
• Injections, Subcutaneous
• Male
• Mice
• Mice, Inbred BALB C
• Mucous Membrane (immunology, virology)
• Pregnancy
• Respirovirus (drug effects, growth & development, immunology)
• Respirovirus Infections (prevention & control)
• Rotavirus (drug effects, growth & development, immunology)
• Rotavirus Infections (prevention & control)
• Titrimetry