HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Interleukin-4-induced macrophage fusion is prevented by inhibitors of mannose receptor activity.

Abstract
A potential role for the macrophage mannose receptor in human monocyte-derived macrophage fusion was explored by testing the effects of previously described inhibitors of its activity on the formation of interleukin-4-induced foreign body giant cells in vitro Giant cell formation was prevented or reduced in the presence of alpha-man-nan and synthetic neoglycoprotein conjugates according to the following pattern of relative inhibition: mannose-bovine serum albumin (BSA) > N-acetylgucosamine-BSA congruent to glucose-BSA. Laminarin (beta-glucan) or galactose-BSA were not inhibitory. Swainsonine and castanospermine, inhibitors of glycoprotein processing that interfere with the arrival of newly synthesized mannose receptors at the cell surface, also attenuated macrophage fusion and the formation of giant cells, whereas another glycosidase inhibitor, deoxymannojirimycin, was without effect. Mannose receptors were confirmed to be specifically up-regulated by interleukin-4 in this culture system and also demonstrated to be present and concentrated at macrophage fusion interfaces. These data suggest that the macrophage mannose receptor may be an essential participant in the mechanism of interleukin-4-induced macrophage fusion and implicate a novel function for this endocytic/phagocytic receptor in mediating foreign body giant cell formation at sites of chronic inflammation.
AuthorsA K McNally, K M DeFife, J M Anderson
JournalThe American journal of pathology (Am J Pathol) Vol. 149 Issue 3 Pg. 975-85 (Sep 1996) ISSN: 0002-9440 [Print] United States
PMID8780401 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Enzyme Inhibitors
  • Indolizines
  • Lectins
  • Lectins, C-Type
  • Mannans
  • Mannose Receptor
  • Mannose-Binding Lectins
  • Receptors, Cell Surface
  • Interleukin-4
  • castanospermine
  • Swainsonine
Topics
  • Cell Fusion (physiology)
  • Cells, Cultured
  • Enzyme Inhibitors (pharmacology)
  • Giant Cells (physiology)
  • Humans
  • Indolizines (pharmacology)
  • Interleukin-4 (pharmacology, physiology)
  • Lectins (antagonists & inhibitors)
  • Lectins, C-Type
  • Macrophages (drug effects, physiology)
  • Mannans (pharmacology)
  • Mannose Receptor
  • Mannose-Binding Lectins
  • Receptors, Cell Surface (antagonists & inhibitors, biosynthesis)
  • Swainsonine (pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: