Abstract |
The effect of cyclophosphamide on the metabolic profile of a mammary carcinoma implanted on the foot of mouse was studied by 31P NMR spectroscopy both in vivo and in perchloric acid extracts. The ratio nucleotide triphosphate:P(i) was significantly elevated in cyclophosphamide treated tumours relative to untreated tumours after 96 h in vivo (p < 0.05). Phosphocreatine:P(i) was similarly elevated from 48 to 168 h (p < 0.01). Resolution of the phosphomonoester peak into two distinct resonances allowed us to estimate the ratio of PME' to phosphocholine (PC), where PME' is a composite peak consisting, in part, of phosphoethanolamine (PE). PME':PC was found to be significantly higher in treated animals relative to control animals in vivo (p < 0.01 from 48 to 168 h). Perchloric acid extract spectra suggest that the increase in PME':PC was in part due to a decrease in PC concentration and also due to an increase in a previously unidentified resonance which was coresonant with PE. Extract data show that there was a significant increase in the concentration of the phosphodiesters, glycerophosphocholine (p < 0.01) and glycerophosphoethanolamine (p < 0.05) in treated relative to control tumours. The changes in the phosphomonoester resonances are qualitatively similar to previously described changes following radiation and suggest that they may be a marker of cell kill or lack of cell growth after antineoplastic therapy.
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Authors | J C Street, U Mahmood, C Matei, J A Koutcher |
Journal | NMR in biomedicine
(NMR Biomed)
Vol. 8
Issue 4
Pg. 149-58
(Jun 1995)
ISSN: 0952-3480 [Print] England |
PMID | 8771089
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antineoplastic Agents, Alkylating
- Phosphates
- Phospholipids
- Phosphorus Isotopes
- Cyclophosphamide
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Topics |
- Adenocarcinoma
(drug therapy, metabolism)
- Animals
- Antineoplastic Agents, Alkylating
(pharmacology)
- Cyclophosphamide
(pharmacology)
- Dose-Response Relationship, Drug
- Drug Screening Assays, Antitumor
- Female
- Magnetic Resonance Spectroscopy
(methods)
- Mammary Neoplasms, Experimental
(drug therapy, metabolism)
- Mice
- Mice, Inbred C3H
- Neoplasm Transplantation
- Phosphates
(metabolism)
- Phospholipids
(metabolism)
- Phosphorus Isotopes
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