A comparison of electrochemiluminescence and flow cytometry for the detection of natural latex-specific human immunoglobulin E.
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| Abstract |
In vitro correlates of type 1 hypersensitivity to natural latex (NL) proteins continue to be limited by both sensitivity and specificity. Methods which have detection limits in the picogram range, namely, radioallergosorbent assays (RAST) and enzyme-linked immunosorbent assays (ELISA), are inadequate for the identification of NL hypersensitivity in certain at-risk groups, such as health care workers. A flow cytometry assay (FCA), previously shown to be comparable to RAST and ELISA in the identification of NL-sensitized pediatric patients with spina bifida, was compared with electrochemiluminescence (ECL) in the evaluation of pediatric patients with spina bifida and NL-sensitized adult health care workers. As with RAST and ELISA, ECL is capable of detecting picogram amounts of specific analyte. The ECL assay detected NL-specific immunoglobulin E (NL-IgE) in three of six health care workers with strong histories of NL hypersensitivity. All six patients were negative by FCA. Further, 2 of 11 spina bifida patients found to be NL-IgE negative by FCA were NL-IgE positive by ECL. These findings suggest that in sensitivity the ECL assay is an improvement over the FCA for the identification of NL-sensitive individuals.
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| Authors | L Kobrynski, L Tanimune, N A Pawlowski, S D Douglas, D E Campbell |
| Journal | Clinical and diagnostic laboratory immunology (Clin Diagn Lab Immunol) Vol. 3 Issue 1 Pg. 42-6 (Jan 1996) ISSN: 1071-412X [Print] United States |
| PMID | 8770502 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't) |
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