Expression and function of autocrine motility factor receptor in human choriocarcinoma.

Abstract

OBJECTIVE:

Our purpose was to determine whether human choriocarcinoma cells express autocrine motility factor receptor (AMF-R) and to study its function in this tumor system.

STUDY DESIGN:

The expression and localization of AMF-R were compared in choriocarcinoma and normal placental trophoblasts using both cell lines and tissue sections. In addition, migratory properties of choriocarcinoma cells and normal placental cells was determined.

RESULTS:

Using immunofluorescence and immunoperoxidase staining, we have detected the expression of AMF-R in choriocarcinoma cells with receptor clustering on the cell surface, while term placenta cells expressed AMF-R less intensely with no receptor clustering. In choriocarcinoma tissues, AMF-R was strongly expressed in malignant cytotrophoblasts cells while adjacent normal villous trophoblast cells and necrotic regions were weakly or negatively stained. Choriocarcinoma cells responded to AMF-R stimulation with increased cell motility, while term placental cells were unresponsive.

CONCLUSION:

Human choriocarcinoma cells express functional cell surface AMF-R in vitro and in choriocarcinoma tissue suggesting that this receptor may play an important role in cancer cell motility.

• Authors: F D Yelian, A Liu, J C Todt, J Lei, F Qureshi, S M Jacques, G Deppe, A Raz
• Journal: Gynecologic oncology (Gynecol Oncol) Vol. 62 Issue 2 Pg. 159-165 (Aug 1996) ISSN: 0090-8258 [Print] United States
• PMID: 8751543 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Receptors, Cytokine
• AMFR protein, human
• Receptors, Autocrine Motility Factor
• Ubiquitin-Protein Ligases

Topics

• Cell Movement
• Choriocarcinoma (metabolism, physiopathology)
• Female
• Fluorescent Antibody Technique
• Gene Expression Regulation, Neoplastic
• Humans
• Immunoenzyme Techniques
• Receptors, Autocrine Motility Factor
• Receptors, Cytokine (biosynthesis, physiology)
• Tumor Cells, Cultured
• Ubiquitin-Protein Ligases