Is P-glycoprotein a sufficient marker for multidrug resistance in vivo? Immunohistochemical staining for P-glycoprotein in children and adult leukemia: correlation with clinical outcome.

Abstract

Seventy-eight patients: 45 children, 33 adults and 27 normal healthy donors were enrolled in the study. Expression of P-glycoprotein (P-gp) was evaluated with three monoclonal antibodies (MAb's) directed to intra-(C219, JSB-1) and extra-cellular (MRK-16) epitopes of P-gp and immunocytochemical (IC) APAAP staining method. Twenty-seven healthy donors peripheral blood mononuclear cells (PBMC) were investigated by means of IC and FACScan analysis. Positive staining for P-gp was detected in 31% children's and 33% adults' leukemia samples. No reactivity of three MAb's was observed with peripheral blood mononuclear cells (PBMC) by means of IC. Flow cytometry analysis with C219 MAb revealed staining for P-gp present on sub-population of lymphocytes and monocytes. P-gp (+) as well as P-gp (-) cases were compared in respect to clinical outcome, FAB classification and blood group. Complete remission (CR) was achieved in 12/14 (85%) children's and 9/11 (81%) adults' P-gp (+) leukemia cases. Within the P-gp (-) leukemia cases CR was observed in 24/29 (82%) and 18/22 (81%), respectively. Partial remission, relapse, resistance and death were noticed in 14% children's and 18% adults' P-gp (+) samples. In P-gp (-) cases these parameters were observed in 17% and 18%, respectively. These results raise the question whether the expression of P-gp can be used as single prognostic marker to detect multidrug resistance (MDR phenomenon) in vivo?

• Authors: S Kaczorowski, M Ochocka, M Kaczorowska, R Aleksandrowicz, M Matysiakl, M Karwacki
• Journal: Leukemia & lymphoma (Leuk Lymphoma) Vol. 20 Issue 1-2 Pg. 143-52 (Dec 1995) ISSN: 1042-8194 [Print] United States
• PMID: 8750636 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ATP Binding Cassette Transporter, Subfamily B, Member 1
• Antibodies, Monoclonal
• Biomarkers
• Epitopes

Topics

• ATP Binding Cassette Transporter, Subfamily B, Member 1 (analysis)
• Adult
• Antibodies, Monoclonal
• Biomarkers
• Blast Crisis
• Cell Line
• Child
• Drug Resistance, Multiple
• Epitopes (analysis)
• Female
• Flow Cytometry
• Humans
• Immunohistochemistry (methods)
• Leukemia, Myeloid, Acute (blood, immunology, pathology)
• Lymphocytes (pathology)
• Lymphoma, Non-Hodgkin (blood, immunology, pathology)
• Male
• Monocytes (pathology)
• Precursor Cell Lymphoblastic Leukemia-Lymphoma (blood, immunology, pathology)
• Treatment Outcome
• Tumor Cells, Cultured