The neurotrophic effects of ebiratide, an analog of ACTH4-9, on cultured septal cells and aged rats.

Abstract

The neurotrophic effects of ebiratide, an ACTH4-9 analog, have been examined using both fetal rat septal cultures and aged rats. The 5-day treatment with ebiratide (10-100 pmol/ml) partially prevented neuronal degeneration that occurred in the cultures in which cells were sparsely plated. Ebiratide (10 pmol/ ml) increased choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activities up to 1.5 and 1.2 times the respective control values in the sub-confluent cultures. AChE cytochemistry of the cultures has shown that ebiratide increased the stained area per cell. Ebiratide subcutaneously administered by constant infusion (10 nmol/body/hr) for 4 weeks elevated ChAT activities in the septum (35% over control), neocortex (79%) and hippocampus (89%) of aged rats. Thus, the present study indicates that ebiratide shares neurotrophic properties which may prove beneficial in the therapy for CNS degenerative disorders, especially Alzheimer's disease.

• Authors: T Matsumoto, S Tsuda, S Nakamura
• Journal: Journal of neural transmission. General section (J Neural Transm Gen Sect) Vol. 100 Issue 1 Pg. 1-15 ( 1995) Austria
• PMID: 8748659 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Peptide Fragments
• Adrenocorticotropic Hormone
• ebiratide
• Choline O-Acetyltransferase
• Acetylcholinesterase

Topics

• Acetylcholinesterase (agonists)
• Adrenocorticotropic Hormone (analogs & derivatives, pharmacology)
• Age Factors
• Amino Acid Sequence
• Animals
• Brain (drug effects, ultrastructure)
• Cell Division
• Cells, Cultured
• Cerebral Cortex (drug effects)
• Choline O-Acetyltransferase (agonists)
• Female
• Hippocampus (drug effects)
• Male
• Molecular Sequence Data
• Peptide Fragments (pharmacology)
• Rats
• Rats, Inbred WKY
• Rats, Sprague-Dawley
• Septum Pellucidum (drug effects, ultrastructure)