A wound- and methyl jasmonate-inducible transcript coding for a myrosinase-associated protein with similarities to an early nodulin.

Abstract	Myrosinase is regarded as a defense-related enzyme in the Brassicaceae and is capable of hydrolyzing glucosinolates into various compounds, some of which are toxic. Several myrosinase isoenzymes exist, and some of them have been found in association with nonmyrosinase proteins. One of these associated proteins, myrosinase-associated protein (MyAP), was purified from seeds of Brassica napus both in complexes with myrosinase and in a free form. MyAP is a glycosylated, 40-kD protein with at least one intramolecular disufide bridge. A monoclonal anti-MyAP antibody precipitated myrosinase activity from B. napus seed extracts and in these complexes both a 65- and a 70-kD myrosinase were present. The subsequent cloning and analysis revealed the existence of a gene family encoding MyAP or MyAP-related protein and that transcripts corresponding to MyAP in nonwounded plants are found predominantly in seeds. At least some members of the gene family exhibited responsiveness toward wounding and methyl jasmonate vapor. MyAP displayed considerable similarity to an early nodulin (ENOD8) from Medicago sativa and to a proline-rich protein (APG), described as another specific, from Arabidopsis thaliana and B. napus. Similarity to expressed sequence tags from both A. thaliana and Oryza sativa has also been found.
Authors	J Taipalensuu, A Falk, L Rask
Journal	Plant physiology (Plant Physiol) Vol. 110 Issue 2 Pg. 483-91 (Feb 1996) ISSN: 0032-0889 [Print] United States
PMID	8742330 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Acetates Cyclopentanes Glycoproteins Intracellular Signaling Peptides and Proteins Membrane Proteins MyAP protein, Brassica napus Oligodeoxyribonucleotides Oxylipins Plant Growth Regulators Plant Proteins RNA, Messenger Recombinant Proteins nodulin methyl jasmonate Glycoside Hydrolases thioglucosidase
Topics	Acetates (pharmacology) Amino Acid Sequence Base Sequence Brassica (genetics, metabolism) Chromatography, Affinity Chromatography, Ion Exchange Cloning, Molecular Cyclopentanes (pharmacology) Electrophoresis, Polyacrylamide Gel Enzyme Induction Genes, Plant Glycoproteins (biosynthesis, chemistry, isolation & purification) Glycoside Hydrolases (metabolism) Intracellular Signaling Peptides and Proteins Membrane Proteins Molecular Sequence Data Molecular Weight Oligodeoxyribonucleotides Oxylipins Plant Growth Regulators (pharmacology) Plant Proteins (biosynthesis, chemistry, isolation & purification) RNA, Messenger (analysis, biosynthesis) Recombinant Proteins (biosynthesis, chemistry, isolation & purification) Sequence Homology, Amino Acid Transcription, Genetic (drug effects) Wounds and Injuries

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