Abstract | OBJECTIVE: DESIGN: 30 steers assigned randomly to 6 treatment groups of 5 steers each: 1, phosphate-buffered saline solution (PBSS; control); 2, PBSS control, fed tylosin (100 mg/steer) daily; 3, inactivated whole-cell culture with oil emulsion adjuvant; 4, culture supernatant (crude toxoid) with oil emulsion adjuvant; 5, semipurified leukotoxoid with oil emulsion adjuvant; and 6, semipurified leukotoxoid with saponin adjuvant. PROCEDURE: Steers were inoculated SC with emulsified antigen or PBSS on days 0 and 21. Blood samples were collected at weekly intervals to monitor serum antileukotoxin antibody titer. On day 42, all steers were challenge exposed intraportally with F necrophorum culture. Three weeks later (day 63), steers were euthanatized and necropsied to examine liver and assess protection. RESULTS: Antileukotoxin antibody titers of all vaccinated groups markedly increased from baseline values, and mean titers of vaccinated groups were higher than those of the control and tylosin-treated groups. Steers vaccinated with culture supernatant with oil emulsion adjuvant or semipurified leukotoxoid with saponin adjuvant had the highest mean antibody titers. All 5 steers in the control group developed liver abscesses. Tylosin feeding did not protect steers challenge exposed with F necrophorum intraportally. CONCLUSIONS: Culture supernatant was more protective than whole-cell culture or semipurified leukotoxin against experimentally induced hepatic abscesses. Partial purification of leukotoxin appeared to reduce its protective immunity.
|
Authors | S Saginala, T G Nagaraja, Z L Tan, K F Lechtenberg, M M Chengappa, K E Kemp, P M Hine |
Journal | American journal of veterinary research
(Am J Vet Res)
Vol. 57
Issue 4
Pg. 483-8
(Apr 1996)
ISSN: 0002-9645 [Print] United States |
PMID | 8712511
(Publication Type: Journal Article)
|
Chemical References |
- Adjuvants, Immunologic
- Anti-Bacterial Agents
- Antibodies, Bacterial
- Bacterial Toxins
- Bacterial Vaccines
- Exotoxins
- leukotoxin
- Tylosin
|
Topics |
- Abscess
(immunology, prevention & control, veterinary)
- Adjuvants, Immunologic
- Animal Feed
- Animals
- Anti-Bacterial Agents
(administration & dosage, therapeutic use)
- Antibodies, Bacterial
(blood)
- Antibody Formation
- Bacterial Toxins
(immunology)
- Bacterial Vaccines
- Cattle
- Cattle Diseases
- Exotoxins
(immunology)
- Fusobacterium Infections
(immunology, prevention & control, veterinary)
- Fusobacterium necrophorum
- Liver
(drug effects, pathology)
- Liver Diseases
(immunology, prevention & control, veterinary)
- Liver Function Tests
- Male
- Neutralization Tests
- Orchiectomy
- Tylosin
(administration & dosage, therapeutic use)
- Vaccination
(veterinary)
|