E-selectin appears in nonischemic tissue during experimental focal cerebral ischemia.

Abstract

BACKGROUND AND PURPOSE:

E-selectin participates in leukocyte-endothelial adhesion and the inflammatory processes that follow focal cerebral ischemia and reperfusion. The temporal and topographical patterns of microvascular E-selectin presentation after experimental focal cerebral ischemia are relevant to microvascular reactivity to ischemia.

METHODS:

The upregulation and fate of E-selectin antigen during 2 hours of middle cerebral artery occlusion (n = 4) and 3 hours of occlusion with reperfusion (1 hour, n = 4; 4 hours, n = 6; 24 hours, n = 6) were evaluated in the nonhuman primate. E-selectin and E:P-selectin immunoreactivities were semiquantitated with the use of computerized light microscopy video imaging and laser confocal microscopy.

RESULTS:

Three patterns of microvascular E-selectin expression, defined by the antibody E-1E4, were confirmed by complete elimination of E-1E4 binding after incubation with soluble recombinant human E-selectin: (1) Low immunoperoxidase intensity was observed in ischemic microvessels at 2 hours of occlusion extending to 4 hours of reperfusion (E-selectin/laminin = 0.32 +/- 0.10). (2) A significant fraction of ischemic microvessels displayed high-intensity E-selectin signal by 24 hours of reperfusion (0.61 +/- 0.17) compared with control and nonischemic tissues (2P < .003). (3) In the contralateral nonischemic basal ganglia and other nonischemic tissues, low but significant E-selectin levels appeared by 24 hours of reperfusion (2P = .0005). The latter were further confirmed by an E:P-selectin immunoprobe.

CONCLUSIONS:

E-selectin antigen is distinctively and significantly upregulated in nonhuman primate brain after focal ischemia and reperfusion. The late appearance of E-selectin in nonischemic cerebral tissues suggests stimulation by transferable factors generated during brain injury.

• Authors: H P Haring, E L Berg, N Tsurushita, M Tagaya, G J del Zoppo
• Journal: Stroke (Stroke) Vol. 27 Issue 8 Pg. 1386-91; discussion 1391-2 (Aug 1996) ISSN: 0039-2499 [Print] United States
• PMID: 8711807 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• E-Selectin
• Fluorescent Dyes

Topics

• Animals
• Antibodies, Monoclonal
• Basal Ganglia (blood supply, chemistry)
• Brain Chemistry (physiology)
• Brain Ischemia (metabolism)
• Disease Models, Animal
• E-Selectin (analysis, immunology)
• Fluorescent Dyes
• Male
• Mice
• Microcirculation
• Papio
• Reperfusion Injury (metabolism)