Molecular analysis of the gene cluster involved in cephalosporin biosynthesis from Lysobacter lactamgenus YK90.

Abstract

Determination of the nucleotide sequence downstream from the Lysobacter lactamgenus pcbC gene encoding isopenicillin N synthase revealed that five open-reading frames (ORF) including the pcbC gene were tightly linked in the same orientation. Each ORF has the remarkable feature of the protein-coding frame in the DNA sequence with a high G+C content. Expression in Escherichia coli and a comparison of the deduced amino acid sequences with published sequences showed that the gene cluster contained a deacetoxycephalosporin C synthetase (DAOCS) gene (cefE), an ORF having homology with the Cephalosporium acremonium DAOCS/deacetylcephalosporin C synthetase gene (cefEF), an isopenicillin N epimerase gene(cefD), and a beta-lactamase gene. The gene order was pcbC-cefE-ORF3-cefD-beta-lactamase.

• Authors: H Kimura, M Izawa, Y Sumino
• Journal: Applied microbiology and biotechnology (Appl Microbiol Biotechnol) Vol. 44 Issue 5 Pg. 589-96 (Jan 1996) ISSN: 0175-7598 [Print] Germany
• PMID: 8703429 (Publication Type: Journal Article)

Chemical References

• Cephalosporins
• Oxidoreductases
• isopenicillin N synthetase

Topics

• Amino Acid Sequence
• Base Sequence
• Cephalosporins (biosynthesis)
• Genes, Bacterial
• Gram-Negative Bacteria (genetics)
• Molecular Sequence Data
• Multigene Family
• Open Reading Frames
• Oxidoreductases (genetics)