A computer-controlled apparatus for measuring and analysing myoclonic jerk activity in guinea pigs.

Abstract

Guinea pigs respond to 5-hydroxytryptophan (5-HTP) with a complex behavioural syndrome which includes distinctive whole-body jerks (myoclonic jerks). These are species-specific, involve all major muscle groups, are highly rhythmic and can occur with relatively high frequency (1-2/s). Current methods of quantifying the response, which rely on observer ratings are unsatisfactory. We report the development of a fully automated, PC-based system for detecting and analysing myoclonic jerks. Floor-mounted accelerometers generate a movement signal and detection is performed by an algorithm based on a detailed analysis of the topography of myoclonic jerk responses. The system can monitor the output from 10 purpose-built test enclosures and it provides for pseudorandom treatment allocation, flexible control of experimental parameters and automated data output to a spreadsheet which generates appropriate graphs, statistical summaries and data analysis. This robust response provides a useful means of exploring the role of novel 5-HT receptor subtypes in guinea-pig behaviour.

• Authors: P S Lloyd, P D Slade, J P Hatcher, J J Hagan
• Journal: Journal of neuroscience methods (J Neurosci Methods) Vol. 64 Issue 2 Pg. 253-62 (Feb 1996) ISSN: 0165-0270 [Print] Netherlands
• PMID: 8699888 (Publication Type: Journal Article)

Chemical References

• Serotonin Antagonists
• Serotonin
• Methysergide

Topics

• Algorithms
• Analysis of Variance
• Animals
• Computers
• Dose-Response Relationship, Drug
• Epilepsies, Myoclonic (chemically induced)
• Guinea Pigs
• Male
• Methysergide (pharmacology)
• Serotonin
• Serotonin Antagonists (pharmacology)
• Software
• Species Specificity